EC Number | General Stability | Organism |
---|---|---|
2.1.3.3 | prolonged dialysis and freeze-thawing causes loss of activity | Lathyrus sativus |
2.1.3.3 | purified enzyme is highly unstable even in the presence of glycerol, dithiothreitol and Mg2+ | Lathyrus sativus |
2.1.3.6 | highly unstable even in the presence of glycerol, dithiothreitol and Mg2+, activity is stabilized in dilute solutions, less than 0.05 mg protein/ml for about 3-4 h at 37°C by 0.25 mg/ml bovine serum albumine | Lathyrus sativus |
2.7.2.2 | all the component activities associated with putrescine synthase are stabilized in dilute solutions, 0.05 mg of protein per ml, for about 3-4 h at 37°C by 0.250 mg/ml bovine serum albumin | Lathyrus sativus |
2.7.2.2 | prolonged dialysis and freeze-thawing, loss of activity | Lathyrus sativus |
2.7.2.2 | purified enzyme is highly unstable even in presence of glycerol, dithiothreitol and Mg2+ | Lathyrus sativus |
3.5.3.12 | highly unstable even in presence of glycerol, dithiothreitol and Mg2+, freeze-thawing and dialysis lead to considerable loss of activity | Lathyrus sativus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.1.3.3 | cytoplasm | - |
Lathyrus sativus | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 55000 | - |
1 * 55000, multifunctional enzyme, SDS-PAGE | Lathyrus sativus |
2.1.3.3 | 56000 | - |
multifunctional enzyme with agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase activity, gel filtration | Lathyrus sativus |
2.1.3.6 | 55000 | - |
1 * 55000, SDS-PAGE | Lathyrus sativus |
2.1.3.6 | 56000 | - |
gel filtration | Lathyrus sativus |
3.5.3.12 | 55000 | - |
gel filtration, PAGE | Lathyrus sativus |
3.5.3.12 | 56000 | - |
gel filtration, Sephadex G-200 | Lathyrus sativus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.2 | ATP + NH3 + CO2 | Lathyrus sativus | multifunctional enzyme, involved in biosynthesis of putrescine | ADP + carbamoyl phosphate | - |
r | |
3.5.3.12 | agmatine + H2O | Lathyrus sativus | - |
N-carbamoylputrescine + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.3.3 | Lathyrus sativus | - |
putrescine synthase has inherent activities of agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase | - |
2.1.3.6 | Lathyrus sativus | - |
- |
- |
2.7.2.2 | Lathyrus sativus | - |
enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithine transcarbamylase and carbamate kinase | - |
3.5.3.12 | Lathyrus sativus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.3.3 | putrescine synthase has inherent activities of agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase, ammonium sulfate, putrescine-Sepharose affinity column | Lathyrus sativus |
2.1.3.6 | MnCl2, ammonium sulfate, putrescine-CH-Sepharose affinity column | Lathyrus sativus |
2.7.2.2 | enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithine transcarbamylase and carbamate kinase | Lathyrus sativus |
3.5.3.12 | - |
Lathyrus sativus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.1.3.6 | carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine | multifunctional enzyme that catalyzes the reactions of EC 2.1.3.3 ornithine carbamoyltransferase, EC 2.7.2.2 carbamate kinase and EC 3.5.3.12 agmatine deiminase, thus acting as putrescine synthase, converting agmatine and ornithine into putrescine and citrulline, respectively | Lathyrus sativus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.3.3 | seedling | - |
Lathyrus sativus | - |
2.1.3.6 | seedling | - |
Lathyrus sativus | - |
2.7.2.2 | seedling | - |
Lathyrus sativus | - |
3.5.3.12 | seedling | - |
Lathyrus sativus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 0.47 | - |
multifunctional enzyme | Lathyrus sativus |
3.5.3.12 | 1.1 | - |
- |
Lathyrus sativus |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.3.3 | 4°C, 48 h, complete loss of activity | Lathyrus sativus |
2.1.3.6 | 4°C, 48 h, complete loss of activity | Lathyrus sativus |
2.7.2.2 | 4°C, 48 h, complete loss of activity | Lathyrus sativus |
3.5.3.12 | 4°C purified enzyme lost all component activities within 48 h | Lathyrus sativus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.3 | carbamoyl phosphate + L-ornithine | enzyme also catalyzes the reactions of EC 2.1.3.6, EC 2.7.2.2 and EC 3.5.3.12, thus acting as putrescine synthase converting agmatine and ornithine into putrescine and citrulline | Lathyrus sativus | phosphate + L-citrulline | - |
r | |
2.1.3.6 | arsenate + N-carbamoylputrescine | - |
Lathyrus sativus | ? | - |
? | |
2.7.2.2 | ADP + carbamoyl phosphate | - |
Lathyrus sativus | ATP + NH3 + CO2 | - |
r | |
2.7.2.2 | ATP + NH3 + CO2 | - |
Lathyrus sativus | ADP + carbamoyl phosphate | - |
r | |
2.7.2.2 | ATP + NH3 + CO2 | multifunctional enzyme, involved in biosynthesis of putrescine | Lathyrus sativus | ADP + carbamoyl phosphate | - |
r | |
3.5.3.12 | agmatine + H2O | - |
Lathyrus sativus | N-carbamoylputrescine + NH3 | - |
? | |
3.5.3.12 | agmatine + H2O | multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase | Lathyrus sativus | N-carbamoylputrescine + NH3 | - |
? | |
3.5.3.12 | agmatine + ornithine + H2O + phosphate | - |
Lathyrus sativus | putrescine + citrulline + NH3 + phosphate | - |
? | |
3.5.3.12 | carbamoyl phosphate + ADP + H2O | - |
Lathyrus sativus | ATP + CO2 + NH3 | - |
r | |
3.5.3.12 | carbamoyl phosphate + ornithine | - |
Lathyrus sativus | citrulline + phosphate | - |
r | |
3.5.3.12 | N-carbamoylputrescine + phosphate | - |
Lathyrus sativus | putrescine + carbamoyl phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.3.3 | monomer | 1 * 55000, multifunctional enzyme, SDS-PAGE | Lathyrus sativus |
2.1.3.6 | monomer | 1 * 55000, SDS-PAGE | Lathyrus sativus |
3.5.3.12 | monomer | 1 * 55000, single polypeptide chain, SDS-PAGE | Lathyrus sativus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.2.2 | putrescine synthase | enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithinetranscarbamylase and carbamate kinase | Lathyrus sativus |
3.5.3.12 | putrescine synthase | - |
Lathyrus sativus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 8 | - |
- |
Lathyrus sativus |
3.5.3.12 | 7.5 | - |
putrescine transcarbamylase and carbamate kinase function | Lathyrus sativus |
3.5.3.12 | 8 | - |
ornithine transcarbamylase function | Lathyrus sativus |
3.5.3.12 | 8.8 | - |
agmatine iminohydrolase function | Lathyrus sativus |