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Literature summary extracted from

  • Knodler, L.A.; Sekyere, E.O.; Stewart, T.S.; Schofield, P.J.; Edwards, M.R.
    Cloning and expression of a prokaryotic enzyme, arginine deiminase, from primitive eukaryote Giardia intestinalis (1998), J. Biol. Chem., 273, 4470-4477.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.6 expression in Escherichia coli Giardia intestinalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.6 agmatine
-
 Giardia intestinalis
3.5.3.6 L-canavanine
-
 Giardia intestinalis
3.5.3.6 L-homoarginine
-
 Giardia intestinalis
3.5.3.6 L-ornithine
-
 Giardia intestinalis
3.5.3.6 putrescine
-
 Giardia intestinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.6 66000
-
 2 * 66000, SDS-PAGE Giardia intestinalis
3.5.3.6 140000
-
 gel filtration Giardia intestinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.6 L-Arg + H2O Giardia intestinalis enzyme of catabolism of Arg to citrulline in the arginine dihydrolase pathway citrulline + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.6 Giardia intestinalis Q27657
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.6 recombinant enzyme Giardia intestinalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.6 36.5
-
 recombinant enzyme Giardia intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.6 L-Arg + H2O
-
 Giardia intestinalis L-citrulline + NH3
-
 ir
3.5.3.6 L-Arg + H2O enzyme of catabolism of Arg to citrulline in the arginine dihydrolase pathway Giardia intestinalis citrulline + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.6 dimer 2 * 66000, SDS-PAGE Giardia intestinalis