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Literature summary extracted from

  • Kimura, M.; Kamakura, T.; Tao, Q.Z.; Kaneko, I.; Yamaguchi, I.
    Cloning of the blasticidin S deaminase from Aspergillus terreus and its use as a selectable marker for Schizosaccharomyces pombe and Pyricularia oryzae (1994), Mol. Gen. Genet., 242, 121-129.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.23 IPTG inducer Aspergillus terreus

Application

EC Number Application Comment Organism
3.5.4.23 molecular biology BSD gene will be useful as a new dominant selectable marker for eukaryotes, first sucessful transformation with a drug resistance gene originating from a eukaryote by selecting for detoxification of the drug Aspergillus terreus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.23 bsd overexpressed in Escherichia coli BL 21 Aspergillus terreus
3.5.4.23 bsr gene employed as marker gene for Escherichia coli, Bacillus subtilis, Nicotiana tabacum, Rhizopus niveus and cultured mammalian cells Bacillus sp. (in: Bacteria)
3.5.4.23 PCR amplification of the BSD gene, cloned from cDNA and expressed in Escherichia coli, also transformed into Schizosaccharomyces pombe and Pyricularia oryzae, P. oryzae could not be transformed with bsr, but with BSD Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.23 0.021
-
 blasticidin S
-
 Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.23 13470
-
 calculated from amino acid sequence Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.23 blasticidin S + H2O Bacillus sp. (in: Bacteria)
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus terreus
-
 deaminohydroxyblasticidin S + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.23 Aspergillus terreus
-
 S-712 (ATCC 28865)
-
3.5.4.23 Bacillus sp. (in: Bacteria)
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.23 blasticidin S + H2O
-
 Bacillus sp. (in: Bacteria) deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus terreus deaminohydroxyblasticidin S + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.23 dimer
-
 Aspergillus terreus