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Literature summary extracted from

  • Fukuda, H.; Kizaki, Y.
    A new transformation system of Saccharomyces cerevisiae with blasticidin S deaminase gene (1999), Biotechnol. Lett., 21, 969-971.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.23 bsd overexpressed in Escherichia coli BL 21 Aspergillus terreus
3.5.4.23 PCR amplification of the BSD gene, cloned from cDNA and expressed in Escherichia coli, also transformed into Schizosaccharomyces pombe and Pyricularia oryzae, P. oryzae could not be transformed with bsr, but with BSD Aspergillus terreus
3.5.4.23 plasmid pAUR123-BSD, transformation of industrial yeast Saccharomyces cerevisiae X-2180AB Aspergillus terreus
3.5.4.23 selectable marker for mammalian cells Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.23 blasticidin S + H2O Aspergillus terreus
-
 deaminohydroxyblasticidin S + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.23 Aspergillus terreus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.23
-
 Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.23 blasticidin S + H2O
-
 Aspergillus terreus deaminohydroxyblasticidin S + NH3
-
 ?