Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rossi, C.A.; Hakim, G.; Solaini, G.
    Purification and properties of pig brain guanine deaminase (1978), Biochim. Biophys. Acta, 526, 235-246.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.3 0.011
-
 guanine
-
 Sus sp.
3.5.4.3 0.33
-
 8-Azaguanine
-
 Sus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.3 50000
-
 2 * 50000, SDS-PAGE Mus musculus
3.5.4.3 50000
-
 2 * 50000, SDS-PAGE Oryctolagus cuniculus
3.5.4.3 50000
-
 2 * 50000, SDS-PAGE Sus sp.
3.5.4.3 55000
-
-
 Rattus norvegicus
3.5.4.3 55000
-
-
 Oryctolagus cuniculus
3.5.4.3 110000
-
-
 Rattus norvegicus
3.5.4.3 110000
-
-
 Oryctolagus cuniculus
3.5.4.3 110000
-
 brain, gel filtration Sus sp.
3.5.4.3 170000
-
-
 Oryctolagus cuniculus
3.5.4.3 207000
-
-
 Oryctolagus cuniculus
3.5.4.3 525000
-
-
 Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.3 guanine + H2O Mus musculus
-
 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O Rattus norvegicus
-
 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O Oryctolagus cuniculus
-
 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O Platyrrhini
-
 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O Ovis ammon
-
 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O Sus sp.
-
 xanthine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.3 Mus musculus
-
 mouse
-
3.5.4.3 Oryctolagus cuniculus
-
 rabbit
-
3.5.4.3 Ovis ammon
-
 sheep
-
3.5.4.3 Platyrrhini
-
-
-
3.5.4.3 Rattus norvegicus
-
 rat
-
3.5.4.3 Sus sp.
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.3
-
 Sus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.3 brain
-
 Mus musculus
-
3.5.4.3 brain
-
 Rattus norvegicus
-
3.5.4.3 brain
-
 Oryctolagus cuniculus
-
3.5.4.3 brain
-
 Sus sp.
-
3.5.4.3 liver
-
 Mus musculus
-
3.5.4.3 liver
-
 Rattus norvegicus
-
3.5.4.3 liver
-
 Oryctolagus cuniculus
-

Storage Stability

EC Number Storage Stability Organism
3.5.4.3 4°C, 0.1 M phosphate buffer, 2 mM 2-mercaptoethanol, pH 7.5, stable for at least 3 months Sus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.3 6-thioguanine + H2O
-
 Oryctolagus cuniculus 6-thioxanthine + NH3
-
 ?
3.5.4.3 6-thioguanine + H2O
-
 Sus sp. 6-thioxanthine + NH3
-
 ?
3.5.4.3 8-azaguanine + H2O
-
 Sus sp. 8-azaxanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Mus musculus xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Rattus norvegicus xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Oryctolagus cuniculus xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Platyrrhini xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Ovis ammon xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Sus sp. xanthine + NH3
-
 ?
3.5.4.3 additional information high specificity towards guanine, 2-amino-6-chloropurine, 8-bromoguanine, 7-methylguanine, thioguanine, guanosine, guanosine 5'-monophosphate, guanilic acid, adenosine and 2-aminopurine are no substrates Sus sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.3 dimer
-
 Rattus norvegicus
3.5.4.3 dimer 2 * 50000, SDS-PAGE Mus musculus
3.5.4.3 dimer 2 * 50000, SDS-PAGE Oryctolagus cuniculus
3.5.4.3 dimer 2 * 50000, SDS-PAGE Sus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.3 5.5 10
-
 Sus sp.