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Literature summary extracted from
- A 5,10-methylenetetrahydrofolate dehydrogenase: 5,10-methenyltetrahydrofolate cyclohydrolase protein from Pisum sativum (1995), Phytochemistry, 39, 1309-1317.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.5 | dihydropteroylmonoglutamate | competitive inhibition | Pisum sativum |  |
| 1.5.1.5 | dihydropteroylpentaglutamate | competitive inhibition | Pisum sativum | |
| 1.5.1.5 | pteroylglutamate | competitive inhibition | Pisum sativum | |
| 1.5.1.5 | pteroylglutamates | - |
Pisum sativum | |
| 3.5.4.9 | dihydrofolate | - |
Pisum sativum | |
| 3.5.4.9 | folic acid | - |
Pisum sativum | |
| 3.5.4.9 | NADP+ | - |
Pisum sativum | |
| 3.5.4.9 | Pyridine nucleotide | - |
Pisum sativum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.5 | 0.011 | - |
NADP+ | - |
Pisum sativum | |
| 1.5.1.5 | 0.021 | - |
tetrahydropteroylmonoglutamate | - |
Pisum sativum | |
| 1.5.1.5 | 0.025 | - |
Tetrahydropteroylpentaglutamate | - |
Pisum sativum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.4.9 | cytosol | - |
Pisum sativum | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 38500 | - |
? * 38500, probably not a heterodimer, SDS-PAGE | Pisum sativum |
| 1.5.1.5 | 58000 | - |
methylenetetrahydrofolate dehydrogenase-methyltetrahydrofolate cyclohydrolase protein, gel filtration | Pisum sativum |
| 3.5.4.9 | 31000 | - |
SDS-PAGE | Escherichia coli |
| 3.5.4.9 | 38500 | - |
SDS-PAGE | Pisum sativum |
| 3.5.4.9 | 40000 | - |
Western blot analysis, cross-reactivity with purified pea enzyme | Vicia faba |
| 3.5.4.9 | 40000 | - |
Western blot analysis, cross-reactivity with purified pea enzyme | Triticum aestivum |
| 3.5.4.9 | 40000 | - |
Western blot analysis, cross-reactivity with purified pea enzyme | Hordeum vulgare |
| 3.5.4.9 | 40000 | - |
Western blot analysis, cross-reactivity with purified pea enzyme | Zea mays |
| 3.5.4.9 | 40000 | - |
Western blot analysis, cross-reactivity with purified pea enzyme | Neurospora sp. |
| 3.5.4.9 | 58000 | - |
gel filtration | Pisum sativum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Vicia faba | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Escherichia coli | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Triticum aestivum | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Hordeum vulgare | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Spinacia oleracea | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Pisum sativum | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Zea mays | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Moorella thermoacetica | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Neurospora sp. | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Gottschalkia acidurici | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Escherichia coli BE | - |
10-formyltetrahydrofolate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.5 | Pisum sativum | - |
L. cv. Homesteader | - |
| 3.5.4.9 | Escherichia coli | - |
bifunctional enzyme | - |
| 3.5.4.9 | Escherichia coli BE | - |
bifunctional enzyme | - |
| 3.5.4.9 | Gottschalkia acidurici | - |
- |
- |
| 3.5.4.9 | Hordeum vulgare | - |
barley | - |
| 3.5.4.9 | Moorella thermoacetica | - |
bifunctional enzyme | - |
| 3.5.4.9 | Neurospora sp. | - |
trifunctional enzyme | - |
| 3.5.4.9 | Pisum sativum | - |
bifunctional enzyme | - |
| 3.5.4.9 | Spinacia oleracea | - |
spinach | - |
| 3.5.4.9 | Triticum aestivum | - |
wheat | - |
| 3.5.4.9 | Vicia faba | - |
bean | - |
| 3.5.4.9 | Zea mays | - |
corn | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.1.5 | co-purification of 5,10-methylenetetrahydrofolate dehydrogenase and 5,10-methenyltetrahydrofolate cyclohydrolase | Pisum sativum |
| 3.5.4.9 | - |
Pisum sativum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.5.1.5 | cotyledon | - |
Pisum sativum | - |
| 1.5.1.5 | leaf | - |
Pisum sativum | - |
| 3.5.4.9 | cotyledon | - |
Pisum sativum | - |
| 3.5.4.9 | leaf | - |
Vicia faba | - |
| 3.5.4.9 | leaf | - |
Triticum aestivum | - |
| 3.5.4.9 | leaf | - |
Hordeum vulgare | - |
| 3.5.4.9 | leaf | - |
Spinacia oleracea | - |
| 3.5.4.9 | leaf | - |
Pisum sativum | - |
| 3.5.4.9 | leaf | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | additional information | - |
- |
Pisum sativum |
| 3.5.4.9 | 12.5 | - |
- |
Pisum sativum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.5.1.5 | -20°C, 1 month, residual activity of 50% | Pisum sativum |
| 1.5.1.5 | 4°C, in the presence of 25% glycerol, 1 month, residual activity of 85% | Pisum sativum |
| 3.5.4.9 | -20°C, 25% glycerol, 20% cyclohydrolase activity remains after 1 month | Pisum sativum |
| 3.5.4.9 | 4°C, 25% glycerol, 60% cyclohydrolase activity remains after 1 month | Pisum sativum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.5 | tetrahydropteroylglutamic acid + NADP+ | - |
Pisum sativum | ? | - |
? | |
| 1.5.1.5 | tetrahydropteroylpentaglutamate + NADP+ | - |
Pisum sativum | ? | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Vicia faba | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Triticum aestivum | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Hordeum vulgare | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Spinacia oleracea | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Pisum sativum | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Zea mays | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Moorella thermoacetica | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Neurospora sp. | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Gottschalkia acidurici | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli BE | 10-formyltetrahydrofolate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.1.5 | homodimer | ? * 38500, probably not a heterodimer, SDS-PAGE | Pisum sativum |
| 3.5.4.9 | dimer | 2 * 31000, SDS-PAGE | Escherichia coli |
| 3.5.4.9 | monomer | 1 * 58000 | Pisum sativum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.4.9 | 5,10-CH+-H4PteGlu cyclohydrolase | - |
Pisum sativum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 65 | - |
- |
Pisum sativum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 6.5 | - |
- |
Pisum sativum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.5 | NADP+ | - |
Pisum sativum | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.5 | 0.003 | - |
dihydropteroylpentaglutamate | varying concentration of tetrahydropteroylmonoglutamate | Pisum sativum | |
| 1.5.1.5 | 0.035 | - |
dihydropteroylmonoglutamate | varying concentration of tetrahydropteroylmonoglutamate | Pisum sativum | |
| 1.5.1.5 | 0.61 | - |
pteroylglutamate | varying concentration of tetrahydropteroylmonoglutamate | Pisum sativum | |
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