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Literature summary extracted from

  • Cheung, E.; D'Ari, L.; Rabinowitz, J.C.; Dyer, D.H.; Huang, J.Y.; Stoddard, B.L.
    Purification, crystallization, and preliminary X-ray studies of a bifunctional 5,10-methenyl/methylene tetrahydrofolate cyclohydrolase/dehydrogenase from Escherichia coli (1997), Proteins, 27, 322-324.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.9 DNA fragment containing the bifunctional enzyme from Escherichia coli BE cloned and transformed into Escherichia coli BL21 for protein expression Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.9 small platelike crystals belong to body centered orthorhombic space group I222 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.9 32000
-
 2 * 32000 Drosophila melanogaster
3.5.4.9 32000
-
 2 * 32000 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Drosophila melanogaster
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Escherichia coli
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Homo sapiens
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Rattus norvegicus
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Saccharomyces cerevisiae
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Escherichia coli BE
-
 10-formyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.9 Drosophila melanogaster
-
 bifunctional enzyme
-
3.5.4.9 Escherichia coli
-
 bifunctional enzyme
-
3.5.4.9 Escherichia coli BE
-
 bifunctional enzyme
-
3.5.4.9 Homo sapiens
-
-
-
3.5.4.9 Rattus norvegicus
-
-
-
3.5.4.9 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Drosophila melanogaster 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Escherichia coli 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Homo sapiens 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Rattus norvegicus 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Saccharomyces cerevisiae 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Escherichia coli BE 10-formyltetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.9 dimer
-
 Saccharomyces cerevisiae
3.5.4.9 dimer 2 * 32000 Drosophila melanogaster
3.5.4.9 dimer 2 * 32000 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.9 8.5
-
-
 Drosophila melanogaster
3.5.4.9 8.5
-
-
 Escherichia coli