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Literature summary extracted from

  • Stevenson, D.E.; Feng, R.; Dumas, F.; Groleau, D.; Mihoc, A.; Storer, A.C.
    Mechanistic and structural studies on Rhodococcus ATCC 39484 nitrilase (1992), Biotechnol. Appl. Biochem., 15, 283-302.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.5.1 synthesis preparing carboxylic acids from readily available nitrile analogues Rhodococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 5,5'-dithiobis(2-nitrobenzoic acid) complete inhibition at 1 mM Rhodococcus sp.
3.5.5.1 benzaldehyde nearly complete inhibition at 10 mM Rhodococcus sp.
3.5.5.1 benzyl bromide strong inhibition at 10 mM Rhodococcus sp.
3.5.5.1 benzylamine 65% inhibition at 10 mM Rhodococcus sp.
3.5.5.1 Hg2+ nearly complete inactivation at 1 mM Rhodococcus sp.
3.5.5.1 phenylacetonitrile 50% inhibition at 10 mM Rhodococcus sp.
3.5.5.1 phenylmethylsulfonyl fluoride 40% inhibition at 1 mM Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 1.5
-
 3-chlorobenzonitrile
-
 Rhodococcus sp.
3.5.5.1 2
-
 4-nitrobenzonitrile
-
 Rhodococcus sp.
3.5.5.1 2.4
-
 3-methylbenzonitrile
-
 Rhodococcus sp.
3.5.5.1 2.6
-
 3-cyanobenzonitrile
-
 Rhodococcus sp.
3.5.5.1 2.7
-
 3-Methoxybenzonitrile
-
 Rhodococcus sp.
3.5.5.1 3.5
-
 3-aminobenzonitrile
-
 Rhodococcus sp.
3.5.5.1 3.8
-
 Benzonitrile
-
 Rhodococcus sp.
3.5.5.1 4.8
-
 3-Nitrobenzonitrile
-
 Rhodococcus sp.
3.5.5.1 8
-
 4-aminobenzonitrile
-
 Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 41500
-
 1 * 41500, SDS-PAGE Rhodococcus sp.
3.5.5.1 46000
-
 gel filtration, aggregation to 560 kDa in the presence of benzonitrile Rhodococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.1 3-cyanopyridine + 2 H2O Rhodococcus sp.
-
 nicotinic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O Rhodococcus sp.
-
 acrylic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O Rhodococcus sp.
-
 benzoic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Rhodococcus sp.
-
 strain ATCC 39484
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 to homogeneity Rhodococcus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.5.1 cell culture
-
 Rhodococcus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.1 84
-
-
 Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 2 phenylacetonitrile + 3 H2O
-
 Rhodococcus sp. phenylacetamide + phenylacetate + NH3 not completely converted to acid, 2% of the product is phenylacetamide ?
3.5.5.1 2-furanocarbonitrile + H2O best substrate Rhodococcus sp. furan-2-carboxylic acid + NH3
-
 ?
3.5.5.1 3-aminobenzonitrile + H2O
-
 Rhodococcus sp. 3-aminobenzoic acid + NH3
-
 ?
3.5.5.1 3-chlorobenzonitrile + H2O
-
 Rhodococcus sp. 3-chlorobenzoic acid + NH3
-
 ?
3.5.5.1 3-cyanobenzonitrile + H2O
-
 Rhodococcus sp. 3-cyanobenzoic acid + NH3
-
 ?
3.5.5.1 3-cyanopyridine + 2 H2O
-
 Rhodococcus sp. nicotinic acid + NH3
-
 ?
3.5.5.1 3-methoxybenzonitrile + H2O
-
 Rhodococcus sp. 3-methoxybenzoic acid + NH3
-
 ?
3.5.5.1 3-nitrobenzonitrile + H2O
-
 Rhodococcus sp. 3-nitrobenzoic acid + NH3
-
 ?
3.5.5.1 3-toluonitrile + H2O
-
 Rhodococcus sp. NH3 + 3-methylbenzoate
-
 ?
3.5.5.1 4-aminobenzonitrile + H2O
-
 Rhodococcus sp. 4-aminobenzoic acid + NH3
-
 ?
3.5.5.1 4-nitrobenzonitrile + H2O
-
 Rhodococcus sp. 4-nitrobenzoic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O
-
 Rhodococcus sp. acrylic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Rhodococcus sp. benzoic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 monomer 1 * 41500, SDS-PAGE Rhodococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.1 40
-
 irreversible inactivation by preincubation above Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 7.5
-
-
 Rhodococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.1 5.5 9.5 active within Rhodococcus sp.
3.5.5.1 5.5 8 stable between Rhodococcus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.5.1 10
-
 phenylacetonitrile
-
 Rhodococcus sp.