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Literature summary extracted from
- Purification and characterization of inorganic pyrophosphatase from Thiobacillus thiooxidans (1977), J. Biochem., 81, 477-483.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.1 | Guanidine HCl | - |
Acidithiobacillus thiooxidans |  |
| 3.6.1.1 | iodoacetamide | - |
Acidithiobacillus thiooxidans | |
| 3.6.1.1 | KCN | - |
Acidithiobacillus thiooxidans | |
| 3.6.1.1 | N-ethylmaleimide | - |
Acidithiobacillus thiooxidans | |
| 3.6.1.1 | NaF | - |
Acidithiobacillus thiooxidans | |
| 3.6.1.1 | NaN3 | - |
Acidithiobacillus thiooxidans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.6.1.1 | cytosol | - |
Acidithiobacillus thiooxidans | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.1 | Co2+ | 25% relative activity compared to Mg2+ | Acidithiobacillus thiooxidans | |
| 3.6.1.1 | Mg2+ | maximum activity | Acidithiobacillus thiooxidans | |
| 3.6.1.1 | Mn2+ | 10.6% relative activity compared to Mg2+ | Acidithiobacillus thiooxidans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 20000 | - |
4 * 20000, SDS-PAGE | Acidithiobacillus thiooxidans |
| 3.6.1.1 | 88000 | - |
SDS-PAGE | Acidithiobacillus thiooxidans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.1 | diphosphate + H2O | Acidithiobacillus thiooxidans | - |
2 phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.1 | Acidithiobacillus thiooxidans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.1 | - |
Acidithiobacillus thiooxidans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 804.1 | - |
- |
Acidithiobacillus thiooxidans |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.6.1.1 | 0°C, purified enzyme, more than 9 months | Acidithiobacillus thiooxidans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.1 | ADP + H2O | 2.6% relative activity compared to diphosphate | Acidithiobacillus thiooxidans | ? | - |
? | |
| 3.6.1.1 | ATP + H2O | 4.2% relative activity compared to diphosphate | Acidithiobacillus thiooxidans | ? | - |
? | |
| 3.6.1.1 | diphosphate + H2O | - |
Acidithiobacillus thiooxidans | 2 phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.6.1.1 | tetramer | 4 * 20000, SDS-PAGE | Acidithiobacillus thiooxidans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 80 | - |
10 min | Acidithiobacillus thiooxidans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 8.8 | - |
- |
Acidithiobacillus thiooxidans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 5.9 | 9.5 | 4.5% and 72.2% relative activity at pH 5.9 and 9.5 respectively | Acidithiobacillus thiooxidans |
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