EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.20 | 4-Pyridinecarboxylic acid | - |
Paracoccus denitrificans | |
4.3.1.20 | EDTA | 0.1 mM almost complete inhibition, activity restored by divalent cations | Paracoccus denitrificans | |
4.3.1.20 | hydroxylamine | - |
Paracoccus denitrificans | |
4.3.1.20 | Maleate | competitive | Paracoccus denitrificans | |
4.3.1.20 | p-chloromercuribenzoate | GSH protects | Paracoccus denitrificans | |
4.3.1.20 | Semicarbazide | - |
Paracoccus denitrificans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.20 | additional information | - |
additional information | Km-value varied with pH, optimum at pH 7.8 | Paracoccus denitrificans | |
4.3.1.20 | 0.43 | - |
erythro-L-beta-hydroxyaspartate | - |
Paracoccus denitrificans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.20 | Ca2+ | restores activity after EDTA treatment | Paracoccus denitrificans | |
4.3.1.20 | Mg2+ | restores activity after EDTA treatment | Paracoccus denitrificans | |
4.3.1.20 | Mn2+ | restores activity after EDTA treatment | Paracoccus denitrificans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.20 | Erythro-3-hydroxyaspartate | Paracoccus denitrificans | beta-hydroxyaspartate pathway | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.1.20 | Paracoccus denitrificans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.1.20 | partial | Paracoccus denitrificans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.3.1.20 | 7.15 | - |
- |
Paracoccus denitrificans |
EC Number | Storage Stability | Organism |
---|---|---|
4.3.1.20 | -15°C, with 0.01M Tris-HCl buffer, pH 8, 1 mM MgCl2 and 0.01 mM pyridoxal 5'-phosphate, 50% loss of activity in 60 days | Paracoccus denitrificans |
4.3.1.20 | 2°C, with 0.01M Tris-HCl buffer, pH 8, 1 mM MgCl2 and 0.01 mM pyridoxal 5'-phosphate, 50% loss of activity in 10 days | Paracoccus denitrificans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.20 | Erythro-3-hydroxyaspartate | beta-hydroxyaspartate pathway | Paracoccus denitrificans | ? | - |
? | |
4.3.1.20 | Erythro-DL-beta-hydroxyaspartate | irreversible, enzyme is highly substrate specific using only the L-isomer | Paracoccus denitrificans | Oxaloacetate + NH3 | - |
? | |
4.3.1.20 | erythro-L-beta-hydroxyaspartate | - |
Paracoccus denitrificans | oxaloacetate + NH3 | - |
? | |
4.3.1.20 | additional information | enzyme does not use threo-DL-beta-hydroxyaspartate, erythro and threo-DL-beta-hydroxy-beta methylaspartate, allo D-isomers of serine, threonine and allothreonine, D and L-aspartate, and isomers of tartrate | Paracoccus denitrificans | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.20 | 9.5 | - |
- |
Paracoccus denitrificans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.20 | 5 | 9 | - |
Paracoccus denitrificans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.20 | 6 | 10 | rapid loss of activity below pH 6 and above pH 10 | Paracoccus denitrificans |
4.3.1.20 | 7.5 | 9.5 | most stable | Paracoccus denitrificans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.20 | pyridoxal 5'-phosphate | activates | Paracoccus denitrificans |