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Literature summary extracted from

  • Gibbs, R.G.; Morris, J.G.
    Purification and properties of erythro-beta-hydroxyaspartate dehydratase from Micrococcus denitrificans (1965), Biochem. J., 97, 547-554.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.20 4-Pyridinecarboxylic acid
-
Paracoccus denitrificans
4.3.1.20 EDTA 0.1 mM almost complete inhibition, activity restored by divalent cations Paracoccus denitrificans
4.3.1.20 hydroxylamine
-
Paracoccus denitrificans
4.3.1.20 Maleate competitive Paracoccus denitrificans
4.3.1.20 p-chloromercuribenzoate GSH protects Paracoccus denitrificans
4.3.1.20 Semicarbazide
-
Paracoccus denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.20 additional information
-
additional information Km-value varied with pH, optimum at pH 7.8 Paracoccus denitrificans
4.3.1.20 0.43
-
erythro-L-beta-hydroxyaspartate
-
Paracoccus denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.20 Ca2+ restores activity after EDTA treatment Paracoccus denitrificans
4.3.1.20 Mg2+ restores activity after EDTA treatment Paracoccus denitrificans
4.3.1.20 Mn2+ restores activity after EDTA treatment Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.20 Erythro-3-hydroxyaspartate Paracoccus denitrificans beta-hydroxyaspartate pathway ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.20 Paracoccus denitrificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.20 partial Paracoccus denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.20 7.15
-
-
Paracoccus denitrificans

Storage Stability

EC Number Storage Stability Organism
4.3.1.20 -15°C, with 0.01M Tris-HCl buffer, pH 8, 1 mM MgCl2 and 0.01 mM pyridoxal 5'-phosphate, 50% loss of activity in 60 days Paracoccus denitrificans
4.3.1.20 2°C, with 0.01M Tris-HCl buffer, pH 8, 1 mM MgCl2 and 0.01 mM pyridoxal 5'-phosphate, 50% loss of activity in 10 days Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.20 Erythro-3-hydroxyaspartate beta-hydroxyaspartate pathway Paracoccus denitrificans ?
-
?
4.3.1.20 Erythro-DL-beta-hydroxyaspartate irreversible, enzyme is highly substrate specific using only the L-isomer Paracoccus denitrificans Oxaloacetate + NH3
-
?
4.3.1.20 erythro-L-beta-hydroxyaspartate
-
Paracoccus denitrificans oxaloacetate + NH3
-
?
4.3.1.20 additional information enzyme does not use threo-DL-beta-hydroxyaspartate, erythro and threo-DL-beta-hydroxy-beta methylaspartate, allo D-isomers of serine, threonine and allothreonine, D and L-aspartate, and isomers of tartrate Paracoccus denitrificans ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.20 9.5
-
-
Paracoccus denitrificans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.20 5 9
-
Paracoccus denitrificans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.3.1.20 6 10 rapid loss of activity below pH 6 and above pH 10 Paracoccus denitrificans
4.3.1.20 7.5 9.5 most stable Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.20 pyridoxal 5'-phosphate activates Paracoccus denitrificans