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Literature summary extracted from

  • Kern, D.; Potier, S.; Boulanger, Y.; Lapointe, J.
    The Monomeric glutamyl-tRNA synthetase of Escherichia coli. Purification and relation between its structural and catalytic properties (1979), J. Biol. Chem., 254, 518-524.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.17 p-hydroxymercuribenzoate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.17 0.00008
-
 tRNAGlu
-
 Escherichia coli
6.1.1.17 0.1
-
 ATP Glu Escherichia coli
6.1.1.17 0.18
-
 ATP
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.17 56000
-
 1 * 56000, SDS-PAGE Escherichia coli
6.1.1.17 58000
-
 electrophoresis in polyacrylamide gels of various concentrations Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.17 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.17
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.17 1.071
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 additional information tRNAGlu-dependent ATP-diphosphate exchange Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.17 monomer 1 * 56000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.17 3.4
-
 tRNAGlu
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.17 6.2
-
 tRNAGlu-dependent ATP-diphosphate exchange Escherichia coli
6.1.1.17 8.6
-
 glutamyl-tRNA formation Escherichia coli