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Literature summary extracted from

  • Yang, V.W.; Jeffries, T.W.
    Purification and properties of xylitol dehydrogenase from the xylose-fermenting yeast Candida shehatae (1990), Appl. Biochem. Biotechnol., 26, 197-206.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.9 0.037
-
 NADH
-
 Scheffersomyces shehatae
1.1.1.9 0.16
-
 D-sorbitol
-
 Scheffersomyces shehatae
1.1.1.9 13.8
-
 xylulose
-
 Scheffersomyces shehatae
1.1.1.9 18.5
-
 xylitol
-
 Scheffersomyces shehatae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.9 cytoplasm
-
 Scheffersomyces shehatae 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.9 40000
-
 2 * 40000, SDS-PAGE Scheffersomyces shehatae
1.1.1.9 82000
-
 gel filtration Scheffersomyces shehatae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.9 Xylitol + NAD+ Scheffersomyces shehatae inducible pathway of xylose catabolism ?
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Scheffersomyces shehatae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.9
-
 Scheffersomyces shehatae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.9 15.95
-
-
 Scheffersomyces shehatae

Storage Stability

EC Number Storage Stability Organism
1.1.1.9 -20°C, stable with 50% v/v glycerol Scheffersomyces shehatae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 D-ribitol + NAD+
-
 Scheffersomyces shehatae D-ribulose + NADH
-
 r
1.1.1.9 D-ribulose + NADH
-
 Scheffersomyces shehatae D-ribitol + NAD+
-
 r
1.1.1.9 D-sorbitol + NAD+ oxidation at about 45% Scheffersomyces shehatae D-fructose + NADH + H+
-
 ?
1.1.1.9 D-xylulose + NADH + H+
-
 Scheffersomyces shehatae xylitol + NAD+
-
 r
1.1.1.9 additional information no oxidation of ribitol Scheffersomyces shehatae ?
-
 ?
1.1.1.9 additional information no oxidation of mannitol Scheffersomyces shehatae ?
-
 ?
1.1.1.9 Xylitol + NAD+ inducible pathway of xylose catabolism Scheffersomyces shehatae ?
-
 r
1.1.1.9 xylitol + NAD+
-
 Scheffersomyces shehatae D-xylulose + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.9 dimer 2 * 40000, SDS-PAGE Scheffersomyces shehatae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.9 7.2
-
 xylitol formation Scheffersomyces shehatae
1.1.1.9 8.6
-
 D-xylulose formation Scheffersomyces shehatae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 additional information no activity with NADP+ Scheffersomyces shehatae
1.1.1.9 additional information at high concentrations of xylitol 1.5% as effective as NAD+ Scheffersomyces shehatae
1.1.1.9 additional information very low activity with NADPH Scheffersomyces shehatae
1.1.1.9 NAD+
-
 Scheffersomyces shehatae
1.1.1.9 NADH
-
 Scheffersomyces shehatae