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Literature summary extracted from

  • Souza, J.M.; Plumbridge, J.A.; Calcagno, M.L.
    N-Acetylglucosamine-6-phosphate deacetylase from Escherichia coli: purification and molecular and kinetic characterization (1997), Arch. Biochem. Biophys., 340, 338-346.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.25 5,5'-dithio-bis(2-nitrobenzoate)
-
 Escherichia coli
3.5.1.25 acetate competitive Escherichia coli
3.5.1.25 N-acetyl-D-glucosamine 6-phosphate substrate inhibition at high concentrations Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.25 0.3
-
 N-acetyl-D-glucosamine 6-phosphate
-
 Escherichia coli
3.5.1.25 20
-
 acetate
-
 Escherichia coli
3.5.1.25 25
-
 glucosamine 6-phosphate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.25 41000
-
 4 * 41000, SDS-PAGE Escherichia coli
3.5.1.25 160000 165000 non-denaturing PAGE, gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.25 Escherichia coli
-
 overproducing strain
-
3.5.1.25 Escherichia coli overproducing
-
 overproducing strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.25
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.25 N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate sequential mechanism with ordered release of products and a slow isomerization of the enzyme-acetate complex Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.25 D-glucosamine 6-phosphate + acetate
-
 Escherichia coli N-acetyl-D-glucosamine 6-phosphate + H2O
-
 r
3.5.1.25 D-glucosamine 6-phosphate + acetate
-
 Escherichia coli overproducing N-acetyl-D-glucosamine 6-phosphate + H2O
-
 r
3.5.1.25 N-acetyl-D-glucosamine 6-phosphate + H2O
-
 Escherichia coli D-glucosamine 6-phosphate + acetate
-
 r
3.5.1.25 N-acetyl-D-glucosamine 6-phosphate + H2O
-
 Escherichia coli overproducing D-glucosamine 6-phosphate + acetate
-
 r

Subunits

EC Number Subunits Comment Organism
3.5.1.25 tetramer 4 * 41000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.25 105
-
 N-acetyl-D-glucosamine 6-phosphate
-
 Escherichia coli