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Literature summary extracted from

  • Cook, A.M.; Beilstein, P.; Grossenbacher, H.; Hutter, R.
    Ring cleavage and degradative pathway of cyanuric acid in bacteria (1985), Biochem. J., 231, 25-30.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.5.1.84 strain D, bovine serum albumin, large amounts, stabilizes, rapid loss of activity can be decreased to 25% with large amounts of bovine serum albumin Pseudomonas sp.
3.5.1.84 strain D, dithiothreitol, 10 mM, stabilizes, rapid loss of activity can be decreased to 25% with 10 mM dithiothreitol Pseudomonas sp.
3.5.1.84 strain D, enzyme very labile during purification Pseudomonas sp.
3.5.1.84 strain D, enzyme very labile, complete loss of activity in a few hours Pseudomonas sp.
3.5.1.84 strain D, glycerol, 1.5 M, stabilizes, rapid loss of activity can be decreased to 25% with 1.5 M glycerol Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.84 DEAE-cellulose strain D, slow inactivation, inactivation accelerated markedly on addition of dithiothreitol Pseudomonas sp.
3.5.1.84 dithiothreitol strain D, accelerates inactivation by DEAE-cellulose markedly Pseudomonas sp.
3.5.1.84 Tris strain D, inactivation Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.15 additional information
-
 additional information strain D, kinetic studies Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.84 biuret + H2O Pseudomonas sp. imidodicarbonic diamide urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O Klebsiella pneumoniae imidodicarbonic diamide urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O Pseudomonas sp. involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O Klebsiella pneumoniae involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O Pseudomonas sp. involved in a pathway by which herbicides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O Klebsiella pneumoniae involved in a pathway by which herbicides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O Pseudomonas sp. second enzyme of degradative pathway of cyanuric acid urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O Klebsiella pneumoniae second enzyme of degradative pathway of cyanuric acid urea + CO2 + NH3
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. 2,4,6-trihydroxy-1,3,5-triazine biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Klebsiella pneumoniae 2,4,6-trihydroxy-1,3,5-triazine biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. 1,3,5-triazine-2,4,6(1H,3H,5H)-trione biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Klebsiella pneumoniae 1,3,5-triazine-2,4,6(1H,3H,5H)-trione biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. 2,4,6-trihydroxy-s-triazine biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Klebsiella pneumoniae 2,4,6-trihydroxy-s-triazine biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. first enzyme of degradative pathway of cyanuric acid biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Klebsiella pneumoniae first enzyme of degradative pathway of cyanuric acid biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Klebsiella pneumoniae involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. herterocyclic ring cleavage biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Klebsiella pneumoniae herterocyclic ring cleavage biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Klebsiella pneumoniae involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Klebsiella pneumoniae 99 2,4,6-trihydroxy-1,3,5-triazine biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Klebsiella pneumoniae 99 1,3,5-triazine-2,4,6(1H,3H,5H)-trione biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Klebsiella pneumoniae 99 2,4,6-trihydroxy-s-triazine biuret + CO2
-
 ?
3.5.2.15 additional information Pseudomonas sp. involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria ?
-
 ?
3.5.2.15 additional information Klebsiella pneumoniae involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria ?
-
 ?
3.5.2.15 additional information Pseudomonas sp. involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine ?
-
 ?
3.5.2.15 additional information Klebsiella pneumoniae involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine ?
-
 ?
3.5.2.15 additional information Klebsiella pneumoniae 99 involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria ?
-
 ?
3.5.2.15 additional information Klebsiella pneumoniae 99 involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.84 Klebsiella pneumoniae
-
-
-
3.5.1.84 Klebsiella pneumoniae 99
-
-
-
3.5.1.84 Pseudomonas sp.
-
 strain D, NRRLB-12228 and strain A, NRRLB-12227
-
3.5.2.15 Klebsiella pneumoniae
-
-
-
3.5.2.15 Klebsiella pneumoniae 99
-
-
-
3.5.2.15 Pseudomonas sp.
-
 strain D, NRRLB-12228 and strain A, NRRLB-12227
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.84 partial, strain D, NRRLB-12228, enzyme very labile during purification Pseudomonas sp.
3.5.2.15 partial, strain D, NRRLB-12228 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.84 additional information
-
-
 Pseudomonas sp.
3.5.1.84 additional information
-
-
 Klebsiella pneumoniae
3.5.2.15 additional information
-
-
 Pseudomonas sp.
3.5.2.15 additional information
-
-
 Klebsiella pneumoniae

Storage Stability

EC Number Storage Stability Organism
3.5.1.84 strain D, rapid complete loss of activity in a few hours Pseudomonas sp.
3.5.2.15 4°C, stable for many days, strain D Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.84 biuret + H2O imidodicarbonic diamide Pseudomonas sp. urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O imidodicarbonic diamide Klebsiella pneumoniae urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Pseudomonas sp. urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Klebsiella pneumoniae urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O involved in a pathway by which herbicides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Pseudomonas sp. urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O involved in a pathway by which herbicides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Klebsiella pneumoniae urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O second enzyme of degradative pathway of cyanuric acid Pseudomonas sp. urea + CO2 + NH3
-
 ?
3.5.1.84 biuret + H2O second enzyme of degradative pathway of cyanuric acid Klebsiella pneumoniae urea + CO2 + NH3
-
 ?
3.5.2.15 cyanuric acid + H2O 2,4,6-trihydroxy-1,3,5-triazine Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O 2,4,6-trihydroxy-1,3,5-triazine Klebsiella pneumoniae biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O 1,3,5-triazine-2,4,6(1H,3H,5H)-trione Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O 1,3,5-triazine-2,4,6(1H,3H,5H)-trione Klebsiella pneumoniae biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O 2,4,6-trihydroxy-s-triazine Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O 2,4,6-trihydroxy-s-triazine Klebsiella pneumoniae biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O heterocyclic ring cleavage Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O heterocyclic ring cleavage Klebsiella pneumoniae biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O first enzyme of degradative pathway of cyanuric acid Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O first enzyme of degradative pathway of cyanuric acid Klebsiella pneumoniae biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Klebsiella pneumoniae biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O herterocyclic ring cleavage Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O herterocyclic ring cleavage Klebsiella pneumoniae biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Klebsiella pneumoniae biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O 2,4,6-trihydroxy-1,3,5-triazine Klebsiella pneumoniae 99 biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O 1,3,5-triazine-2,4,6(1H,3H,5H)-trione Klebsiella pneumoniae 99 biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O 2,4,6-trihydroxy-s-triazine Klebsiella pneumoniae 99 biuret + CO2
-
 ?
3.5.2.15 additional information involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Pseudomonas sp. ?
-
 ?
3.5.2.15 additional information involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Klebsiella pneumoniae ?
-
 ?
3.5.2.15 additional information involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Pseudomonas sp. ?
-
 ?
3.5.2.15 additional information involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Klebsiella pneumoniae ?
-
 ?
3.5.2.15 additional information involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Klebsiella pneumoniae 99 ?
-
 ?
3.5.2.15 additional information involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Klebsiella pneumoniae 99 ?
-
 ?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.84 additional information
-
 strain D, essentially inactive at pH 9.5 Pseudomonas sp.