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Literature summary extracted from

  • Pazzagli, L.; Ikram, U.K.; Liguri, G.; Taddei, N.; Gentilini, A.; Cecchi, C.; Manao, G.; Cappugi, G.
    Purification, kinetic properties and primary structure of bovine erythrocyte acylphosphatase (1993), Ital. J. Biochem., 42, 233-245.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.7 2,3-diphosphoglycerate erythrocyte isoenzyme, competitive inhibition, Ki: 0.24 mM Bos taurus
3.6.1.7 3-phosphoglycerate erythrocyte isoenzyme, competitive inhibition, Ki: 1.70 mM Bos taurus
3.6.1.7 Cl- erythrocyte isoenzyme, competitive inhibition, Ki: 18.0 mM Bos taurus
3.6.1.7 phosphate erythrocyte isoenzyme, competitive inhibition, Ki: 0.41 mM Bos taurus
3.6.1.7 phosphoenolpyruvate erythrocyte isoenzyme, competitive inhibition, Ki: 0.21 mM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.7 additional information
-
 additional information kinetic studies Bos taurus
3.6.1.7 additional information
-
 additional information kinetic characterization, kinetic data Bos taurus
3.6.1.7 0.12
-
 benzoyl phosphate erythrocyte enzyme Bos taurus
3.6.1.7 4.23
-
 acetyl phosphate erythrocyte enzyme Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.7 cytoplasm
-
 Bos taurus 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.7 additional information Bos taurus since acylphosphatase hydrolyzes the high-energy phosphorylated compounds its action is probably involved in the regulation of cell energy metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.7 Bos taurus
-
 bovine
-
3.6.1.7 Bos taurus
-
 two isoenzymes: muscular isoenzyme, IM, and erythrocyte isoenzyme, IE
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.6.1.7 side-chain modification erythrocyte isoenzyme, NH2-terminus is acetylated Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.7 erythrocyte form isoenzyme Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.7 erythrocyte erythrocyte isoenzyme Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.7 6800
-
 erythrocyte form isoenzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.7 (Ca2+-Mg2+)-ATPase phosphorylated intermediate + H2O
-
 Bos taurus ?
-
 ?
3.6.1.7 1,3-diphosphoglycerate + H2O
-
 Bos taurus 3-phosphoglycerate + phosphate
-
 ?
3.6.1.7 acetyl phosphate + H2O
-
 Bos taurus acetate + phosphate
-
 ?
3.6.1.7 acylphosphate + H2O specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Bos taurus carboxylate + phosphate
-
 ?
3.6.1.7 benzoyl phosphate + H2O
-
 Bos taurus benzoate + phosphate
-
 ?
3.6.1.7 carbamoyl phosphate + H2O
-
 Bos taurus carbamate + phosphate
-
 ?
3.6.1.7 additional information since acylphosphatase hydrolyzes the high-energy phosphorylated compounds its action is probably involved in the regulation of cell energy metabolism Bos taurus ?
-
 ?
3.6.1.7 Na+/K+-ATPase phosphoenzyme intermediate + H2O
-
 Bos taurus ?
-
 ?
3.6.1.7 succinoyl phosphate + H2O
-
 Bos taurus succinate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.7 More two isoenzymes in all tissues, organs, and cell types checked: 1. muscular isoenzyme, IM, and 2. erythrocyte isoenzyme, IE, on the basis of the cell-type in which they are mostly represented and from which they are purified for the first time Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.7 25
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.7 117
-
 benzoyl phosphate
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.7 5.2
-
 erythrocyte isoenzyme Bos taurus
3.6.1.7 5.3
-
 assay at Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.7 additional information
-
 erythrocyte isoenzyme, retains activity over a broad range of pH values Bos taurus