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Literature summary extracted from

  • Buenz, P.V.; Falchetto, R.; Cook, A.M.
    Purification of two isofunctional hydrolases (EC 3.7.1.8) in the degradative pathway for dibenzofuran in Sphingomonas sp. strain RW1 (1993), Biodegradation, 4, 171-178.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.8 29000
-
 1 * 29000, isoenzyme H2, SDS-PAGE Sphingomonas sp.
3.7.1.8 31000
-
 1 * 31000, isoenzyme H1, SDS-PAGE Sphingomonas sp.
3.7.1.8 37000 40000 isoenzyme H1, gel filtration, non-denaturing PAGE Sphingomonas sp.
3.7.1.8 41000 48000 isoenzyme H2, gel filtration, non-denaturing PAGE Sphingomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.8 additional information Sphingomonas sp. enzyme is involved in the degradative pathway for dibenzofuran ?
-
 ?
3.7.1.8 additional information Sphingomonas sp. RW1 enzyme is involved in the degradative pathway for dibenzofuran ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.8 Sphingomonas sp.
-
 2 isofunctional hydrolases H1 and H2
-
3.7.1.8 Sphingomonas sp. RW1
-
 2 isofunctional hydrolases H1 and H2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.8 2 isofunctional hydrolases H1 and H2 Sphingomonas sp.

Storage Stability

EC Number Storage Stability Organism
3.7.1.8 -20°C or -70°C, buffer containing 30% glycerol, stable for 1 week Sphingomonas sp.
3.7.1.8 4°C, 48 h, 10% loss of isoenzyme H1 activity, 20% loss of isoenzyme H2 activity Sphingomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.8 2-hydroxy-6-oxo-6-(2-hydroxyphenyl)hexa-2,4-dienoate + H2O
-
 Sphingomonas sp. 2-oxopent-4-enoate + salicylate
-
 ?
3.7.1.8 2-hydroxy-6-oxo-6-(2-hydroxyphenyl)hexa-2,4-dienoate + H2O
-
 Sphingomonas sp. RW1 2-oxopent-4-enoate + salicylate
-
 ?
3.7.1.8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Sphingomonas sp. benzoate + 2-oxopent-4-enoate
-
 ?
3.7.1.8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Sphingomonas sp. RW1 benzoate + 2-oxopent-4-enoate
-
 ?
3.7.1.8 additional information enzyme is involved in the degradative pathway for dibenzofuran Sphingomonas sp. ?
-
 ?
3.7.1.8 additional information enzyme is involved in the degradative pathway for dibenzofuran Sphingomonas sp. RW1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.8 monomer 1 * 31000, isoenzyme H1, SDS-PAGE Sphingomonas sp.
3.7.1.8 monomer 1 * 29000, isoenzyme H2, SDS-PAGE Sphingomonas sp.