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Literature summary extracted from

  • Bronnenmeier, K.; Meissner, H.; Stocker, S.; Staudenbauer, W.L.
    alpha-D-Glucuronidases from the xylanolytic thermophiles Clostridium stercorarium and Thermoanaerobacterium saccharolyticum (1995), Microbiology, 141, 2033-2040.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.139 CuCl2
-
 Thermoanaerobacterium saccharolyticum
3.2.1.139 CuCl2
-
 Thermoclostridium stercorarium
3.2.1.139 ZnCl2 no inhibition Thermoanaerobacterium saccharolyticum
3.2.1.139 ZnCl2
-
 Thermoclostridium stercorarium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.139 intracellular
-
 Thermoanaerobacterium saccharolyticum 5622
-
3.2.1.139 intracellular
-
 Thermoclostridium stercorarium 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.139 71000
-
 2 * 71000, SDS-PAGE Thermoanaerobacterium saccharolyticum
3.2.1.139 72000
-
 1 * 72000 + 1 * 76000, SDS-PAGE Thermoclostridium stercorarium
3.2.1.139 76000
-
 1 * 72000 + 1 * 76000, SDS-PAGE Thermoclostridium stercorarium
3.2.1.139 118000
-
 gel filtration Thermoanaerobacterium saccharolyticum
3.2.1.139 124000
-
 gel filtration Thermoclostridium stercorarium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.139 Thermoanaerobacterium saccharolyticum
-
-
-
3.2.1.139 Thermoclostridium stercorarium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.139
-
 Thermoanaerobacterium saccharolyticum
3.2.1.139
-
 Thermoclostridium stercorarium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.139 1.647
-
-
 Thermoclostridium stercorarium
3.2.1.139 9.6
-
-
 Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.139 4-O-methyl-glucuronosyl-xylotriose + H2O
-
 Thermoanaerobacterium saccharolyticum 4-O-methyl-glucuronate + xylotriose
-
 ?
3.2.1.139 4-O-methyl-glucuronosyl-xylotriose + H2O
-
 Thermoclostridium stercorarium 4-O-methyl-glucuronate + xylotriose
-
 ?
3.2.1.139 additional information no activity with p-nitrophenyl-alpha-D-glucuronoside Thermoanaerobacterium saccharolyticum ?
-
 ?
3.2.1.139 additional information no activity with p-nitrophenyl-alpha-D-glucuronoside Thermoclostridium stercorarium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.139 dimer 2 * 71000, SDS-PAGE Thermoanaerobacterium saccharolyticum
3.2.1.139 dimer 1 * 72000 + 1 * 76000, SDS-PAGE Thermoclostridium stercorarium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.139 50
-
-
 Thermoanaerobacterium saccharolyticum
3.2.1.139 60
-
-
 Thermoclostridium stercorarium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.139 50
-
-
 Thermoanaerobacterium saccharolyticum
3.2.1.139 50
-
 pH 6.0, 4 h, stable Thermoclostridium stercorarium
3.2.1.139 60
-
 half-life: 14 h Thermoclostridium stercorarium
3.2.1.139 60
-
 half-life: 2-3 h Thermoanaerobacterium saccharolyticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.139 6
-
-
 Thermoanaerobacterium saccharolyticum
3.2.1.139 6
-
-
 Thermoclostridium stercorarium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.139 5 8 at least 40% of maximal activity at pH-values ranging from 5 to 8 Thermoanaerobacterium saccharolyticum
3.2.1.139 5 8 at least 40% of maximal activity at pH-values ranging from 5 to 8 Thermoclostridium stercorarium