Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Boulter, J.R.; Gielow, W.O.
    Properties of D-arabinose isomerase purified from two strains of Escherichia coli (1973), J. Bacteriol., 113, 687-696.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.3 Cd2+
-
 Escherichia coli
5.3.1.3 Dulcitol
-
 Escherichia coli
5.3.1.3 L-arabitol
-
 Escherichia coli
5.3.1.3 ribitol
-
 Escherichia coli
5.3.1.3 sorbitol
-
 Escherichia coli
5.3.1.25 Cd2+
-
 Escherichia coli
5.3.1.25 Dulcitol
-
 Escherichia coli
5.3.1.25 L-arabitol
-
 Escherichia coli
5.3.1.25 ribitol
-
 Escherichia coli
5.3.1.25 sorbitol
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.3 42
-
 L-fucose E. coli B/r Escherichia coli
5.3.1.3 45
-
 L-fucose E. coli K-12 Escherichia coli
5.3.1.3 170
-
 D-arabinose E. coli B/r Escherichia coli
5.3.1.3 280
-
 D-arabinose E. coli K-12 Escherichia coli
5.3.1.25 42
-
 L-fucose E. coli B/r Escherichia coli
5.3.1.25 45
-
 L-fucose E. coli K-12 Escherichia coli
5.3.1.25 170
-
 D-arabinose E. coli B/r Escherichia coli
5.3.1.25 280
-
 D-arabinose E. coli K-12 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.3 Co2+ stimulates Escherichia coli
5.3.1.3 Mn2+ stimulates Escherichia coli
5.3.1.25 Co2+ stimulates Escherichia coli
5.3.1.25 Mn2+ stimulates Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.3 84600
-
 4 * 84600, Escherichia coli B/r, high speed sedimentation of enzyme dissociated into subunits by protonation at pH 2 or dialysis against buffer containing 8 M urea Escherichia coli
5.3.1.3 90900
-
 4 * 90900, Escherichia coli K-12, high speed sedimentation of enzyme dissociated into subunits by protonation at pH 2 or dialysis against buffer containing 8 M urea Escherichia coli
5.3.1.3 342000
-
 strain B/r, high-speed equilibrium sedimentation Escherichia coli
5.3.1.3 355000
-
 strain K-12, high-speed equilibrium sedimentation Escherichia coli
5.3.1.25 84600
-
 4 * 84600, Escherichia coli B/r, high speed sedimentation of enzyme dissociated into subunits by protonation at pH 2 or dialysis against buffer containing 8 M urea Escherichia coli
5.3.1.25 90900
-
 4 * 90900, Escherichia coli K-12, high speed sedimentation of enzyme dissociated into subunits by protonation at pH 2 or dialysis against buffer containing 8 M urea Escherichia coli
5.3.1.25 342000
-
 strain B/r, high-speed equilibrium sedimentation Escherichia coli
5.3.1.25 355000
-
 strain K-12, high-speed equilibrium sedimentation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.3 Escherichia coli
-
-
-
5.3.1.3 Escherichia coli B/r
-
-
-
5.3.1.25 Escherichia coli
-
-
-
5.3.1.25 Escherichia coli B/r
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.1.3 culture condition:arabinose-grown cell L-fucose induced culture of strain K-12 and D-arabinose-induced culture of strain B/r Escherichia coli
-
5.3.1.3 culture condition:D-arabinose-induced cell strain B/r Escherichia coli
-
5.3.1.3 culture condition:L-fucose-induced cell strain K-12 Escherichia coli
-
5.3.1.25 culture condition:arabinose-grown cell L-fucose induced culture of strain K-12 and D-arabinose-induced culture of strain B/r Escherichia coli
-
5.3.1.25 culture condition:D-arabinose-induced cell strain B/r Escherichia coli
-
5.3.1.25 culture condition:L-fucose-induced cell strain K-12 Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.3 63.3
-
 Escherichia coli K-12 Escherichia coli
5.3.1.3 63.7
-
 Escherichia coli B/r Escherichia coli
5.3.1.25 63.3
-
 Escherichia coli K-12 Escherichia coli
5.3.1.25 63.7
-
 Escherichia coli B/r Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.3 D-arabinose
-
 Escherichia coli D-ribulose
-
 ?
5.3.1.3 D-arabinose
-
 Escherichia coli B/r D-ribulose
-
 ?
5.3.1.3 L-Fucose
-
 Escherichia coli L-Fuculose
-
 ?
5.3.1.3 L-Fucose
-
 Escherichia coli B/r L-Fuculose
-
 ?
5.3.1.25 D-arabinose
-
 Escherichia coli D-ribulose
-
 ?
5.3.1.25 D-arabinose
-
 Escherichia coli B/r D-ribulose
-
 ?
5.3.1.25 L-Fucose
-
 Escherichia coli L-Fuculose
-
 ?
5.3.1.25 L-Fucose
-
 Escherichia coli B/r L-Fuculose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.3 tetramer 4 * 84600, Escherichia coli B/r, high speed sedimentation of enzyme dissociated into subunits by protonation at pH 2 or dialysis against buffer containing 8 M urea Escherichia coli
5.3.1.3 tetramer 4 * 90900, Escherichia coli K-12, high speed sedimentation of enzyme dissociated into subunits by protonation at pH 2 or dialysis against buffer containing 8 M urea Escherichia coli
5.3.1.25 tetramer 4 * 84600, Escherichia coli B/r, high speed sedimentation of enzyme dissociated into subunits by protonation at pH 2 or dialysis against buffer containing 8 M urea Escherichia coli
5.3.1.25 tetramer 4 * 90900, Escherichia coli K-12, high speed sedimentation of enzyme dissociated into subunits by protonation at pH 2 or dialysis against buffer containing 8 M urea Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.3 8 10
-
 Escherichia coli
5.3.1.25 8 10
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.3 7 10 7.0: sharp decrease in activity below, 8.0-10.0: maximal activity Escherichia coli
5.3.1.25 7 10 7.0: sharp decrease in activity below, 8.0-10.0: maximal activity Escherichia coli