EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.1.2.2 | mutant E317Q | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.1.2.2 | E317Q | E317Q with 3400fold reduced turnover number for (R)-mandelate and 29000fold reduced turnover number for (S)-mandelate. E317Q mutant enzyme does not catalyze detectable elimination of Br- from either enantiomer of p-(bromomethyl)mandelate. E317Q mutant enzyme is irreversibly inactivated by racemic alpha-phenylglycidate at a rate comparable to that measured for wild type enzyme | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.1.2.2 | DL-alpha-Phenylglycidate | wild type enzyme and mutant E317Q | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.2.2 | 0.4 | - |
(R)-mandelate | wild type enzyme | Pseudomonas putida | |
5.1.2.2 | 2.4 | - |
(R)-mandelate | E317Q mutant | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.2.2 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.2.2 | wild type and mutant E317Q | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.2.2 | D-mandelate | - |
Pseudomonas putida | L-mandelate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.2.2 | 0.012 | - |
(S)-Mandelate | mutant E317Q | Pseudomonas putida | |
5.1.2.2 | 350 | - |
(S)-Mandelate | wild type enzyme | Pseudomonas putida | |
5.1.2.2 | 500 | - |
(R)-mandelate | wild type enzyme | Pseudomonas putida |