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Literature summary extracted from

  • Eichhorn, E.; Van der Ploeg, J.R.; Kertesz, M.A.; Leisinger, T.
    Characterization of alpha-ketoglutarate-dependent taurine dioxygenase from Escherichia coli (1997), J. Biol. Chem., 272, 23031-23036.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.17 ascorbate 50% increase in activity at 0.2-0.8 mM Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.17
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.17 Co2+ inhibits activity by 80-95% at 0.01-0.05 mM Escherichia coli
1.14.11.17 Cu2+ inhibits activity by 80-95% at 0.01-0.05 mM Escherichia coli
1.14.11.17 EDTA complete inactivation Escherichia coli
1.14.11.17 Zn2+ inhibits activity by 80-95% at 0.01-0.05 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.17 0.011
-
 2-oxoglutarate
-
 Escherichia coli
1.14.11.17 0.055
-
 taurine
-
 Escherichia coli
1.14.11.17 0.145
-
 MOPS
-
 Escherichia coli
1.14.11.17 0.485
-
 1,3-dioxo-2-isoindolineethanesulfonic acid
-
 Escherichia coli
1.14.11.17 0.59
-
 pentanesulfonic acid
-
 Escherichia coli
1.14.11.17 1.49
-
 butanesulfonic acid
-
 Escherichia coli
1.14.11.17 1.51
-
 hexanesulfonic acid
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.17 Fe2+ maximal activation between 0.005 and 0.150 mM Escherichia coli
1.14.11.17 additional information Mg2+, Ca2+, Mn2+ or Ni2+ can not replace iron Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.17 32410
-
 calculation from gene sequence Escherichia coli
1.14.11.17 37400
-
 estimated by SDS-PAGE Escherichia coli
1.14.11.17 81000
-
 gel filtration on Superose 6 and Superose 12 HR Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.17 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.17 two-step purification from overexpressing Escherichia coli to apparent homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.17 0.012
-
 strain MC4100 grown in sulfate-free minimal medium containing 0.25 mM taurine as sulfur source Escherichia coli
1.14.11.17 1.64
-
 purified enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.14.11.17 -20°C, phosphate buffer, 16% glycerol, 10 weeks, activity increases 4-fold Escherichia coli
1.14.11.17 -20°C, phosphate buffer, without glycerol, 3 weeks, more than 50% loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.17 1,3-dioxo-2-isoindolineethanesulfonic acid + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + ? + succinate + CO2
-
 ?
1.14.11.17 butanesulfonic acid + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + butanal + succinate + CO2
-
 ?
1.14.11.17 hexanesulfonic acid + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + hexanal + succinate + CO2
-
 ?
1.14.11.17 MOPS + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + ? + succinate + CO2
-
 ?
1.14.11.17 pentanesulfonic acid + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + pentanal + succinate + CO2
-
 ?
1.14.11.17 taurine + 2-oxoglutarate + O2 no substrates are methanesulfonic acid, ethanesulfonic acid, isethionic acid, 2-bromoethanesulfonic acid, L-cysteic acid, sulfosuccinate, 4-aminobenzenesulfonic acid, 2-(4-pyridyl)ethanesulfonic acid, N-phenyltaurine Escherichia coli sulfite + aminoacetaldehyde + succinate + CO2
-
 ?
1.14.11.17 taurine + alpha-ketoadipate + O2 alpha-ketoadipate is less active than 2-oxoglutarate, no activity with pyruvate, alpha-ketobutyrate, alpha-ketovalerate, alpha-ketocaproate, alpha-ketoisovalerate and oxalacetat Escherichia coli sulfite + aminoacetaldehyde + pentan-1,5-dioic acid + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.17 dimer 2 * 37400, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.11.17 30
-
 incubation at 30°C leads to rapid inactivation, effect is enhanced by ascorbate and not due to oxidation of the enzyme-bound ferrous iron Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.17 6.9
-
-
 Escherichia coli