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Literature summary extracted from
- Purification and characterization of the NADH:ferredoxinBPH oxidoreductase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400 (1998), Arch. Microbiol., 170, 106-112.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.12.18 | 0.058 | - |
NADH | recombinant, purified NADH: ferredoxin oxidoreductase component | Pseudomonas sp. |  |
| 1.14.12.18 | 0.156 | - |
NADPH | recombinant, purified NADH: ferredoxin oxidoreductase component | Pseudomonas sp. | |
| 1.18.1.3 | 0.058 | - |
NADH | reaction with 2,6-dichlorophenolindophenol | Pseudomonas sp. | |
| 1.18.1.3 | 0.156 | - |
NADPH | reaction with 2,6-dichlorophenolindophenol | Pseudomonas sp. | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.18 | Fe2+ | - |
Pseudomonas sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.18 | 41500 | - |
NADH: ferredoxin component, gel filtration with Superose 6 | Pseudomonas sp. |
| 1.14.12.18 | 43600 | - |
NADH: ferredoxin component, SDS-PAGE | Pseudomonas sp. |
| 1.18.1.3 | 41500 | - |
NADH-ferredoxin oxidoreductase component of biphenyl 2,3-dioxygenase, gel filtration | Pseudomonas sp. |
| 1.18.1.3 | 43600 | - |
1 * 43600, NADH-ferredoxin oxidoreductase component of biphenyl 2,3-dioxygenase, SDS-PAGE | Pseudomonas sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.12.18 | Pseudomonas sp. | - |
- |
- |
| 1.14.12.18 | Pseudomonas sp. LB400 | - |
- |
- |
| 1.18.1.3 | Pseudomonas sp. | - |
- |
- |
| 1.18.1.3 | Pseudomonas sp. | - |
NADH-ferredoxin oxidoreductase component of biphenyl 2,3-dioxygenase | - |
| 1.18.1.3 | Pseudomonas sp. LB400 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.12.18 | purification of the NADH: ferredoxin oxidoreductase component | Pseudomonas sp. |
| 1.18.1.3 | NADH-ferredoxin oxidoreductase component of biphenyl 2,3-dioxygenase | Pseudomonas sp. |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.12.18 | -75°C, 4 months, 10% loss of activity | Pseudomonas sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.18 | biphenyl + NAD(P)H + O2 | - |
Pseudomonas sp. | cis-biphenyl 2,3-dihydrodiol + NAD(P)+ | - |
? | |
| 1.14.12.18 | biphenyl + NAD(P)H + O2 | - |
Pseudomonas sp. LB400 | cis-biphenyl 2,3-dihydrodiol + NAD(P)+ | - |
? | |
| 1.18.1.3 | 2 ferricyanide + NADH | - |
Pseudomonas sp. | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| 1.18.1.3 | 2 ferricyanide + NADH | - |
Pseudomonas sp. LB400 | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| 1.18.1.3 | 2 ferricytochrome c + NADH | - |
Pseudomonas sp. | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
| 1.18.1.3 | 2 ferricytochrome c + NADH | - |
Pseudomonas sp. LB400 | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
| 1.18.1.3 | NADH + oxidized 2,6-dichlorophenolindophenol + H+ | - |
Pseudomonas sp. | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.18.1.3 | NADH + oxidized 2,6-dichlorophenolindophenol + H+ | - |
Pseudomonas sp. LB400 | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.18.1.3 | NADH + oxidized nitro blue tetrazolium + H+ | - |
Pseudomonas sp. | NAD+ + reduced nitro blue tetrazolium | - |
? | |
| 1.18.1.3 | NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Pseudomonas sp. | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.18.1.3 | NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Pseudomonas sp. LB400 | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.18.1.3 | monomer | 1 * 43600, NADH-ferredoxin oxidoreductase component of biphenyl 2,3-dioxygenase, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.18 | 32 | - |
NADH: ferredoxin oxidoreductase component | Pseudomonas sp. |
| 1.18.1.3 | 32 | - |
reduction of 2,6-dichlorophenolindophenol | Pseudomonas sp. |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.18.1.3 | additional information | - |
no activity detected at 5°C or at 55°C. Activity at 50°C is 15.2% of maximal activity | Pseudomonas sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.18 | 5 | 55 | NADH: ferredoxin oxidoreductase component: no activity at 5 or 55°C, 15.2% of control activity at 50°C | Pseudomonas sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.18 | 7.2 | - |
NADH: ferredoxin oxidoreductase component | Pseudomonas sp. |
| 1.18.1.3 | 7.2 | - |
reduction of 2,6-dichlorophenolindophenol | Pseudomonas sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.18 | Ferredoxin | - |
Pseudomonas sp. | |
| 1.18.1.3 | FAD | contains 0.89 mol of FAD per mol of enzyme | Pseudomonas sp. | |
| 1.18.1.3 | NADH | - |
Pseudomonas sp. | |
| 1.18.1.3 | NADPH | - |
Pseudomonas sp. | |
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