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Literature summary extracted from

  • Nguyen, L.B.; Shefer, S.; Salen, G.; Ness, G.; Tanaka, R.D.; Packin, V.; Thomas, P.; Shore, V.; Batta, A.
    Purification of cholesterol 7alpha-hydroxylase from human and rat liver and production of inhibiting polyclonal antibodies (1990), J. Biol. Chem., 265, 4541-4546.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.23 microsome
-
 Homo sapiens
-
-
1.14.14.23 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.23 Homo sapiens
-
-
-
1.14.14.23 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.23
-
 Homo sapiens
1.14.14.23
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.23 liver
-
 Homo sapiens
-
1.14.14.23 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.23 0.00015
-
-
 Homo sapiens
1.14.14.23 0.01
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.23 cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.23 cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Rattus norvegicus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.23 heme cytochrome P-450 dependent enzyme Homo sapiens
1.14.14.23 heme cytochrome P-450 dependent enzyme Rattus norvegicus
1.14.14.23 NADPH
-
 Homo sapiens
1.14.14.23 NADPH
-
 Rattus norvegicus