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Literature summary extracted from

  • Sloane, D.L.; So, O.Y.; Leung, R; Scarafia, L.E.; Saldou, N.; Jarnagin, K.; Swinney, D.C.
    Cloning and functional expression of the cDNA encoding rat lanosterol 14alpha-demethylase (1995), Gene, 161, 243-248.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.14.154 medicine target for cholesterol-lowering drugs Rattus norvegicus
1.14.14.154 pharmacology target for cholesterol-lowering drugs Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.154 cDNA, cloning and functional expression Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.154 azalanstat specific inhibitor, IC50 less than 0.000002 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.154 55050
-
 amino acid analysis Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Rattus norvegicus lanosta-8,24-dien-3beta-ol 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Rattus norvegicus 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.154 additional information Rattus norvegicus enzyme of cholesterol biosynthesis ?
-
 ?
1.14.14.154 additional information Rattus norvegicus enzyme for regulation of cholesterol biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.154 Rattus norvegicus Q64654
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.154
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.14.154 a 14alpha-methylsteroid + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = a DELTA14-steroid + formate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O regulation Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.154 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 lanosta-8,24-dien-3beta-ol Rattus norvegicus 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol Rattus norvegicus 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.154 additional information enzyme of cholesterol biosynthesis Rattus norvegicus ?
-
 ?
1.14.14.154 additional information enzyme for regulation of cholesterol biosynthesis Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.154 rLDM rat lanosterol 14alpha-demethylase Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.154 cytochrome P450
-
 Rattus norvegicus
1.14.14.154 heme heme-thiolate enzyme Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.14.154 0.000002
-
 specific inhibitor, IC50 less than 0.000002 mM Rattus norvegicus azalanstat