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Literature summary extracted from

  • Suzuki, K.; Sanga, K.i.; Chikaoka, Y.; Itagaki, E.
    Purification and properties of cytochrome P-450 (P-450lun) catalyzing steroid 11beta-hydroxylation in Curvularia lunata (1993), Biochim. Biophys. Acta, 1203, 215-223.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.15.4 11-deoxycortisol, partially stabilizes Curvularia lunata
1.14.15.4 glycerol, partially stabilizes Curvularia lunata
1.14.15.4 GSH, partially stabilizes Curvularia lunata
1.14.15.4 PMSF, partially stabilizes Curvularia lunata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.4 CO strongly Curvularia lunata
1.14.15.4 HgCl2 0.2 mM, 50% inhibition Curvularia lunata
1.14.15.4 ketoconazole
-
 Curvularia lunata
1.14.15.4 Metyrapone
-
 Curvularia lunata
1.14.15.4 PCMB 0.1 mM, 50% inhibition Curvularia lunata
1.14.15.4 sodium cholate 0.2% effect nearly 20% inhibition Curvularia lunata
1.14.15.4 Stilbestrol
-
 Curvularia lunata
1.14.15.4 sulfhydryl reagents strongly Curvularia lunata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.4 additional information
-
 additional information lipids such as DLPC required Curvularia lunata
1.14.15.4 0.077
-
 11-deoxycortisol 11beta-hydroxylation Curvularia lunata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.4 60000
-
 x * 60000, SDS-PAGE Curvularia lunata

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.4 Curvularia lunata
-
 Boedijn NRRL 2380
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.4 Triton X-100, sodium cholate, column chromatographies Curvularia lunata

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.4 a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O one bifunctional cytochrome P-450, 11beta-/14alpha-hydroxylation Curvularia lunata
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.4 mycelium
-
 Curvularia lunata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.4 additional information
-
 dependent on DLPC concentration, with 0.05 mM maximum activity, 2fold increase with addition of 15% glycerol, 0.6 mM GSH, 0.01 mM FAD and 0.01 mM FMN Curvularia lunata

Storage Stability

EC Number Storage Stability Organism
1.14.15.4 -80°C, purified, 200 mM sodium phosphate buffer, pH 8.0, 0.2 mM 11-deoxycortisol, 20% glycerol, 0.1 mM GSH, 0.1% sodium cholate, several days without loss of activity Curvularia lunata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.4 11-deoxycortisol + reduced adrenal ferredoxin + O2 11beta-hydroxylase activity Curvularia lunata cortisol + oxidized adrenal ferredoxin + H2O
-
 ?
1.14.15.4 11-deoxycortisol + reduced adrenal ferredoxin + O2 14alpha-hydroxylase activity Curvularia lunata 14alpha-hydroxy-11-deoxycortisol + oxidized adrenal ferredoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.4 ? x * 60000, SDS-PAGE Curvularia lunata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.4 35
-
 30 mM sodium phosphate buffer Curvularia lunata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.15.4 30
-
 30 min, 30 mM sodium phosphate buffer, pH 8.0, decrease to 30%, improved to 45-70% by addition of 1 mM GSH, 0.2 mM 11-deoxycortisol, 0.5 mM PMSF or 20% glycerol Curvularia lunata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.4 3.45
-
 11-deoxycortisol 11beta-hydroxylation Curvularia lunata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.4 7.4
-
 30 mM sodium phosphate buffer Curvularia lunata

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.4 NADPH NADPH-linked cytochrome P-450 reductase Curvularia lunata