Any feedback?
Literature summary extracted from
- Mitochondrial cytochrome P-450sec. Mechanism of electron transport by adrenodoxin (1980), J. Biol. Chem., 255, 3057-3061.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.6 | adrenodoxin | oxidized form, high affinity to P-450scc, inhibits side chain cleavage by competition with reduced form | Bos taurus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.15.6 | 0.0004 | - |
reduced adrenodoxin | free | Bos taurus | |
| 1.14.15.6 | 0.00056 | - |
reduced adrenodoxin | +/-0.00004, complex between adrenodoxin and adrenodoxin reductase | Bos taurus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.15.6 | mitochondrial inner membrane | integral to membrane | Bos taurus | 5743 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.6 | cholesterol + 6 reduced adrenodoxin + 3 O2 | Bos taurus | first step in biosynthesis of all steroid hormones | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.6 | Bos taurus | - |
- |
- |
| 1.18.1.6 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.6 | affinity chromatography | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.15.6 | adrenal cortex | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.6 | cholesterol + 6 reduced adrenodoxin + 3 O2 | first step in biosynthesis of all steroid hormones | Bos taurus | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? | |
| 1.14.15.6 | cholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
| 1.14.15.6 | cholesterol + reduced adrenodoxin + O2 + H+ | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
r | |
| 1.14.15.6 | additional information | suggested shuttle mechanism in which adrenodoxin transfers electrons between adrenodoxin reductase and cytochrome P-450. No ternary adrenodoxin-adrenodoxin reductase-P-450 complex | Bos taurus | ? | - |
? | |
| 1.14.15.6 | additional information | reduction of adrenodoxin must be rate-limiting step | Bos taurus | ? | - |
? | |
| 1.18.1.6 | oxidized adrenodoxin + NADPH + H+ | - |
Bos taurus | reduced adrenodoxin + NADP+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.18.1.6 | adrenodoxin reductase | - |
Bos taurus |
| 1.18.1.6 | FLP | - |
Bos taurus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.6 | 37 | - |
assay at | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.6 | 7.2 | - |
assay at | Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.6 | adrenodoxin | transports electrons from adrenodoxin reductase to cytochrome P-450 by shuttling | Bos taurus | |
| 1.14.15.6 | NADPH | NADPH-linked adrenodoxin reductase | Bos taurus | |
| 1.18.1.6 | FAD | - |
Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
