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Literature summary extracted from

  • Hanukoglu, I.; Privalle, C.T.; Jefcoate, C.R.
    Mechanisms of ionic activation of adrenal mitochondrial cytochromes P-450scc and P-45011beta (1981), J. Biol. Chem., 256, 4329-4335.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.6 Phospholipid inclusion of P-450 into vesicles Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.6 adrenodoxin oxidized form, high affinity to P-450scc, inhibits side chain cleavage by competition with reduced form Bos taurus
1.14.15.6 Ca2+ CaCl2 inhibits side chain cleavage activity at 100 mM NaCl, 50% inhibition with 0.07 mM in Tween 20 and 0.03 mM for vesicle-incorporated, Ca2+ does not affect binding of either cholesterol of oxidized adrenodoxin to P-450scc Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 mitochondrial inner membrane adrenodoxin reductase and adrenodoxin are peripheral proteins on matrix side of inner membrane, P-450scc is integral membrane protein Bos taurus 5743
-
1.14.15.6 mitochondrial inner membrane integral to membrane Bos taurus 5743
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.6 Ca2+ ineffective activator Bos taurus
1.14.15.6 Ca2+ particulary but also other metal ions modulate adrenodoxin binding to adrenodoxin reductase and P-450, therefore activation of cholesterol side chain cleavage and adrenodoxin reduction Bos taurus
1.14.15.6 Mg2+ increased MgCl2 concentrations continue to increase side chain cleavage activity even after all adrenodoxin is reduced Bos taurus
1.14.15.6 additional information increase of side chain cleavage activity from 50 to 100 mM NaCl, higher concentrations cause a decrease, total loss at 300 mM Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.6 DEAE-cellulose, adrenodoxin-Sepharose Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.6 adrenal cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
1.14.15.6 additional information cholesterol side chain cleavage activity is dependent on free reduced adrenodoxin Bos taurus ?
-
 ?
1.14.15.6 additional information suggested shuttle mechanism in which adrenodoxin transfers electrons between adrenodoxin reductase and cytochrome P-450. No ternary adrenodoxin-adrenodoxin reductase-P-450 complex Bos taurus ?
-
 ?
1.14.15.6 additional information dissociation of oxidized adrenodoxin from P-450scc must be a potential rate-limiting factor Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.6 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.6 7.2
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin transports electrons from adrenodoxin reductase to cytochrome P-450 by shuttling Bos taurus
1.14.15.6 NADPH NADPH-linked adrenodoxin reductase Bos taurus