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Literature summary extracted from

  • Shikita, M.; Hall, P.F.
    Cytochrome P-450 from bovine adrenocortical mitochondria: an enzyme for the side chain cleavage of cholesterol. I. Purification and properties (1973), J. Biol. Chem., 248, 5598-5604.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.6 glycerol 5-20% concentration, 20-50% increase of enzyme activity Bos taurus
1.14.15.6 Lipid from adrenal mitochondria accelerates activity Bos taurus

General Stability

EC Number General Stability Organism
1.14.15.6 glycerol, 50%, stabilizes Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.6 0.012
-
 20alpha-hydroxycholesterol
-
 Bos taurus
1.14.15.6 0.19
-
 cholesterol
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 mitochondrial inner membrane electron carriers and P-450 liquid-bound within mitochondrion Bos taurus 5743
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.6 800000
-
 exclusion chromatography Bos taurus
1.14.15.6 850000
-
 sedimentation equilibrium analysis Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.6 cholesterol + 6 reduced adrenodoxin + 3 O2 Bos taurus first step in biosynthesis of all steroid hormones pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.6 cholic acid extraction, ammonium sulfate precipitation, DEAE-cellulose, hydroxylapatite and gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.6 adrenal cortex
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
1.14.15.6 -20°C, 50 mM potassium phosphate buffer, pH 7.0, 50% glycerol, 3 months Bos taurus
1.14.15.6 5°C, 50 mM potassium phosphate buffer, pH 7.0, without glycerol, 21-43% decrease of activity within 30 days, completely lost in 4 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 20alpha-hydroxycholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
1.14.15.6 cholesterol + 6 reduced adrenodoxin + 3 O2 first step in biosynthesis of all steroid hormones Bos taurus pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
 ?
1.14.15.6 cholesterol + reduced adrenodoxin + O2 without demonstrable accumulation of putative intermediates 20alpha-hydroxycholesterol and 20alpha,22-dehydroxycholesterol Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.6 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.6 6.8
-
 for cholesterol and 20alpha-hydroxycholesterol, 75 mM potassium phosphate buffer Bos taurus
1.14.15.6 7
-
 assay at Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.15.6 6 7.5 less than 50% of maximal activity below and above range Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin
-
 Bos taurus
1.14.15.6 heme 8 heme groups per molecule P-450 Bos taurus
1.14.15.6 NADPH
-
 Bos taurus