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Literature summary extracted from

  • Takemori, S.; Sukara, K.; Hashimoto, K.; Hashimoto, M.; Sato, H.; Gomi, T.; Katagiri, M.
    Purification of cytochrome P-450 from bovine adrenocortical mitochondria by an aniline-Sepharose and the properties (1975), Biochem. Biophys. Res. Commun., 63, 588-593.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 mitochondrial inner membrane
-
 Bos taurus 5743
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.6 46000
-
 4 * 46000, sedimentation equilibrium analysis after guanidine treatment and SDS-PAGE Bos taurus
1.14.15.6 200000
-
 sedimentation equilibrium analysis Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.6 ammonium sulfate precipitation, affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.6 adrenal cortex
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
1.14.15.6 -80°C, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.01% cholate, a few days without substantial loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
1.14.15.6 additional information
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.6 tetramer 4 * 46000, sedimentation equilibrium analysis after guanidine treatment and SDS-PAGE Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin
-
 Bos taurus
1.14.15.6 NADPH NADPH-linked adrenodoxin reductase Bos taurus