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Literature summary extracted from

  • Rowell, P.; Diez, J.; Apte, S.K.; Stewart, W.D.P.
    Molecular heterogeneity of ferredoxin:NADP+ oxidoreductase from the cyanobacterium Anabaena cylindrica (1981), Biochim. Biophys. Acta, 657, 507-516.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.0045 0.0046 reduced ferredoxin ferredoxin-dependent cytochrome c reductase activity Anabaena cylindrica
1.18.1.2 0.01 0.016 reduced 2,6-dichlorophenolindophenol NADPH-2,6-dichlorophenol-indophenol diaphorase activity Anabaena cylindrica
1.18.1.2 0.011 0.035 NADPH ferredoxin-dependent cytochrome c reductase activity Anabaena cylindrica
1.18.1.2 0.033 0.062 NADPH NADPH-2,6-dichlorophenol indophenol diaphorase activity Anabaena cylindrica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 33300 37000 SDS-PAGE, gel filtration, monomer Anabaena cylindrica
1.18.1.2 70000
-
 SDS-PAGE, dimer Anabaena cylindrica

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Anabaena cylindrica
-
 cyanobacterium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.2 9.4
-
 fraction 1 Anabaena cylindrica
1.18.1.2 28
-
 fraction 4 Anabaena cylindrica
1.18.1.2 39.4
-
 fraction 2 Anabaena cylindrica
1.18.1.2 53.9
-
 fraction 3 Anabaena cylindrica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 additional information enzyme has also little NADP-2,6-dichlorophenol indophenol diaphorase activity Anabaena cylindrica ?
-
 ?
1.18.1.2 additional information enzyme has also ferredoxin dependent cytochrome c reductase activity Anabaena cylindrica ?
-
 ?
1.18.1.2 NADPH + oxidized ferredoxin
-
 Anabaena cylindrica NADP+ + reduced ferredoxin
-
 ?
1.18.1.2 reduced 2,6-dichlorophenolindophenol + NADP+
-
 Anabaena cylindrica oxidized 2,6-dichlorophenolindophenol + NADPH + H+
-
 ?
1.18.1.2 reduced ferredoxin + NADP+
-
 Anabaena cylindrica oxidized ferredoxin + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 dimer
-
 Anabaena cylindrica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.2 7.4 7.8 ferredoxin-dependent cytochrome c reductase activity Anabaena cylindrica
1.18.1.2 10.1
-
 NADPH-2,6-dichlorophenol-indophenol diaphorase activity Anabaena cylindrica

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 flavin
-
 Anabaena cylindrica