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Literature summary extracted from

  • Preiss, J.; Ashwell, G.
    Polygalacturonic acid metabolism in bacteria. II. Formation and metabolism of 3-deoxy-D-glycero-2,5-hexodiulosonic acid (1963), J. Biol. Chem., 238, 1577-1583.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.127 1 mM NAD+ or NADP+ increase stability Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.127 2-dehydro-3-deoxy-D-gluconate + NAD+ Pseudomonas sp. irreversible at pH 7.0 (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.127 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.127 partial Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.127 cell culture
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.127 19.5
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.127 0-3°C, 1 mM NAD+ or NADP+, 50-67% retaining activity after 5 weeks Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.127 2-dehydro-3-deoxy-D-gluconate + NAD+ irreversible at pH 7.0 Pseudomonas sp. (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.127 7 8
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.127 NAD+
-
 Pseudomonas sp.
1.1.1.127 NADP+
-
 Pseudomonas sp.