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Literature summary extracted from

  • Niehaus, W.G.; Dilts, R.P.
    Purification and characterization of mannitol dehydrogenase from Aspergillus parasiticus (1982), J. Bacteriol., 151, 243-250.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.138 Cd2+ more potent inhibitor than Zn2+, reversible Aspergillus parasiticus
1.1.1.138 Cu2+ inactivation Aspergillus parasiticus
1.1.1.138 KCl half Vmax/Km at less than 0.1 M due to general ionic strength effect Aspergillus parasiticus
1.1.1.138 KNO3 half Vmax/Km at less than 0.1 M due to general ionic strength effect Aspergillus parasiticus
1.1.1.138 NaCl half Vmax/Km at less than 0.1 M due to general ionic strength effect Aspergillus parasiticus
1.1.1.138 NADP+ Ki: 0.014 mM Aspergillus parasiticus
1.1.1.138 Zn2+ reversible inhibition Aspergillus parasiticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.138 0.015
-
 NADP+
-
 Aspergillus parasiticus
1.1.1.138 100
-
 D-mannitol
-
 Aspergillus parasiticus
1.1.1.138 190
-
 D-arabinitol
-
 Aspergillus parasiticus
1.1.1.138 560
-
 D-glucitol
-
 Aspergillus parasiticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.138 32000
-
 4 * 32000, SDS-PAGE Aspergillus parasiticus
1.1.1.138 140000
-
 gel filtration Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.138 D-mannitol + NADP+ Aspergillus parasiticus substrate must have a primary hydroxyl group at C1, an R hydroxyl at C2, an R hydroxyl at C3, an R hydroxyl at C4 and either an R or S or primary hydroxyl at C5 D-fructose + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.138 Aspergillus parasiticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.138 to homogeneity, chromatography techniques, affinity chromatography Aspergillus parasiticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.138 mycelium
-
 Aspergillus parasiticus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.138 130
-
-
 Aspergillus parasiticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.138 1-deoxy-6-methyl-D-glucitol + NADP+ poorer substrate than D-glucitol Aspergillus parasiticus 1-deoxy-6-methyl-D-glucose + NADPH
-
 ?
1.1.1.138 1-deoxy-6-methyl-D-mannitol + NADP+ poorer substrate than D-mannitol Aspergillus parasiticus 1-deoxy-6-methyl-D-fructose + NADPH
-
 ?
1.1.1.138 1-deoxy-D-glucitol + NADP+ poorer substrate than glucitol Aspergillus parasiticus 1-deoxy-D-glucose + NADPH
-
 ?
1.1.1.138 1-deoxy-D-mannitol + NADP+ poorer substrate than D-mannitol Aspergillus parasiticus 1-deoxy-D-fructose + NADPH
-
 ?
1.1.1.138 D-arabinitol + NADP+ substrate must have a primary hydroxyl group at C1, an R hydroxyl at C2, an R hydroxyl at C3, an R hydroxyl at C4 and either an R or S or primary hydroxyl at C5 Aspergillus parasiticus D-xylulose + NADPH + H+
-
 ?
1.1.1.138 D-glucitol + NADP+
-
 Aspergillus parasiticus D-glucose + NADPH + H+
-
 r
1.1.1.138 D-mannitol + 3-acetylpyridine adenine dinucleotide phosphate 7.5% of the activity compared to NADP+ Aspergillus parasiticus D-fructose + 3-acetylpyridine adenine dinucleotide phosphate(H)
-
 ?
1.1.1.138 D-mannitol + NAD+ less than 0.05% of the activity compared to NADP+ Aspergillus parasiticus D-fructose + NADH + H+
-
 ?
1.1.1.138 D-mannitol + NADP+ substrate must have a primary hydroxyl group at C1, an R hydroxyl at C2, an R hydroxyl at C3, an R hydroxyl at C4 and either an R or S or primary hydroxyl at C5 Aspergillus parasiticus D-fructose + NADPH + H+
-
 ?
1.1.1.138 D-mannitol + thio-NADP+ 30% of the activity compared to NADP+ Aspergillus parasiticus D-fructose + thio-NADPH
-
 ?
1.1.1.138 D-sorbitol + NADP+
-
 Aspergillus parasiticus D-sorbose + NADPH + H+
-
 ?
1.1.1.138 L-sorbitol + NADP+ substrate must have a primary hydroxyl group at C1, an R hydroxyl at C2, an R hydroxyl at C3, an R hydroxyl at C4 and either an R or S or primary hydroxyl at C5 Aspergillus parasiticus L-sorbose + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.138 tetramer 4 * 32000, SDS-PAGE Aspergillus parasiticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.138 6 9.5 95% of its maximal activity for 5 min Aspergillus parasiticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.138 NADP+ specific for NADP+ Aspergillus parasiticus