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Literature summary extracted from

  • Leissing, N.; Mc Guinness, E.T.
    Rapid affinity purification and properties of rat liver sorbitol dehydrogenase (1978), Biochim. Biophys. Acta, 524, 254-261.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.14 0.1 mg/ml bovine serum albumin stabilizes at -20°C Rattus norvegicus
1.1.1.14 0.1 mg/ml bovine serum albumin stabilizes at room temperature Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.14 0.35
-
 D-sorbitol
-
 Rattus norvegicus
1.1.1.14 110
-
 D-fructose
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.14 95000
-
 gel filtration Rattus norvegicus
1.1.1.14 97000
-
 disc gel electrophoresis Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.14
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.14 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.14 7.47
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.1.1.14 -20°C, 70% loss of activity after 1 week, 0.1 mg/ml bovine serum albumin stabilizes Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-fructose + NADH + H+
-
 Rattus norvegicus D-sorbitol + NAD+
-
 ?
1.1.1.14 D-mannitol + NAD+
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 D-ribitol + NAD+
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 Rattus norvegicus D-fructose + NADH + H+
-
 ?
1.1.1.14 D-xylitol + NAD+
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 L-iditol + NAD+
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 L-sorbose + NADH
-
 Rattus norvegicus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.14 22
-
 5 h, stable Rattus norvegicus
1.1.1.14 60
-
 10 min, 65% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 5.9
-
 D-fructose reduction Rattus norvegicus
1.1.1.14 8.1 8.5 sorbitol oxidation Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.14 5 7 at pH 5.0 and pH 7.0 about 60% of maximal activity, fructose reduction Rattus norvegicus
1.1.1.14 7.5 9.5 at pH 7.5 and pH 9.5 about 70% of maximal activity, sorbitol oxidation Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+ specific for Rattus norvegicus
1.1.1.14 NADH specific for Rattus norvegicus
1.1.1.14 NADP+ cannot replace NAD+ Rattus norvegicus