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Literature summary extracted from

  • Rizzi, M.; Harwart, K.; Erlemann, P.; Bui-Thanh, N.A.; Dellweg, H.
    Purification and properties of the NAD+-xylitol-dehydrogenase from the yeast Pichia stipitis (1989), J. Ferment. Bioeng., 67, 20-24.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.14 ADP 2 mM, 72% activity Scheffersomyces stipitis
1.1.1.14 AMP 2 mM, 83% activity Scheffersomyces stipitis
1.1.1.14 ATP 2 mM, 54% activity Scheffersomyces stipitis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.14 0.16
-
NAD+
-
Scheffersomyces stipitis
1.1.1.14 26
-
D-xylitol
-
Scheffersomyces stipitis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.14 32000
-
2 * 32000, SDS-PAGE Scheffersomyces stipitis
1.1.1.14 63000
-
gel filtration Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.14 xylitol + NAD+ Scheffersomyces stipitis
-
D-xylulose + NADH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Scheffersomyces stipitis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.14
-
Scheffersomyces stipitis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.14 58.1
-
-
Scheffersomyces stipitis

Storage Stability

EC Number Storage Stability Organism
1.1.1.14 -60°C, 5 mM 2-mercaptoethanol, several months Scheffersomyces stipitis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-fructose + NADH + H+
-
Scheffersomyces stipitis D-sorbitol + NAD+
-
?
1.1.1.14 D-ribitol + NAD+
-
Scheffersomyces stipitis ?
-
?
1.1.1.14 D-sorbitol + NAD+
-
Scheffersomyces stipitis D-fructose + NADH + H+
-
?
1.1.1.14 D-xylitol + NAD+
-
Scheffersomyces stipitis ?
-
?
1.1.1.14 D-xylulose + NADH
-
Scheffersomyces stipitis ?
-
?
1.1.1.14 erythritol + NAD+
-
Scheffersomyces stipitis ?
-
?
1.1.1.14 L-iditol + NAD+
-
Scheffersomyces stipitis ?
-
?
1.1.1.14 L-sorbose + NADH
-
Scheffersomyces stipitis ?
-
?
1.1.1.14 xylitol + NAD+
-
Scheffersomyces stipitis D-xylulose + NADH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.14 dimer 2 * 32000, SDS-PAGE Scheffersomyces stipitis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.14 35
-
-
Scheffersomyces stipitis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.14 15 50 50% of maximal activity at 15°C, 40% of maximal activity at 50°C Scheffersomyces stipitis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 9
-
sorbitol oxidation Scheffersomyces stipitis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.14 7 10 40% of maximal activity at 7 and 70% of maximal activity at 10, sorbitol oxidation Scheffersomyces stipitis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+ specific for Scheffersomyces stipitis
1.1.1.14 NADH specific for Scheffersomyces stipitis