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Literature summary extracted from

  • Adachi, O.; Toyama, H.; Theeragool, G.; Lotong, N.; Matsushita, K.
    Crystallization and properties of NAD-dependent D-sorbitol dehydrogenase from Gluconobacter suboxydans IFO 3257 (1999), Biosci. Biotechnol. Biochem., 63, 1589-1595.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.14 0.21
-
 NAD+
-
 Gluconobacter oxydans
1.1.1.14 0.28
-
 NADH
-
 Gluconobacter oxydans
1.1.1.14 5
-
 D-sorbitol
-
 Gluconobacter oxydans
1.1.1.14 20
-
 D-fructose
-
 Gluconobacter oxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.14 26000
-
 4 * 26000, SDS-PAGE Gluconobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Gluconobacter oxydans
-
-
-
1.1.1.14 Gluconobacter oxydans IFO3257
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.14
-
 Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.14 136.5
-
-
 Gluconobacter oxydans

Storage Stability

EC Number Storage Stability Organism
1.1.1.14 4°C several days Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-fructose + NADH + H+
-
 Gluconobacter oxydans D-sorbitol + NAD+
-
 ?
1.1.1.14 D-fructose + NADH + H+
-
 Gluconobacter oxydans IFO3257 D-sorbitol + NAD+
-
 ?
1.1.1.14 D-mannitol + NAD+ low activity Gluconobacter oxydans ?
-
 ?
1.1.1.14 D-mannitol + NAD+ low activity Gluconobacter oxydans IFO3257 ?
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 Gluconobacter oxydans D-fructose + NADH + H+
-
 r
1.1.1.14 D-sorbitol + NAD+
-
 Gluconobacter oxydans IFO3257 D-fructose + NADH + H+
-
 r
1.1.1.14 D-xylitol + NAD+
-
 Gluconobacter oxydans ?
-
 ?
1.1.1.14 D-xylitol + NAD+
-
 Gluconobacter oxydans IFO3257 ?
-
 ?
1.1.1.14 D-xylulose + NADH
-
 Gluconobacter oxydans ?
-
 ?
1.1.1.14 D-xylulose + NADH
-
 Gluconobacter oxydans IFO3257 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.14 tetramer 4 * 26000, SDS-PAGE Gluconobacter oxydans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.14 40
-
 30 min, stable Gluconobacter oxydans
1.1.1.14 50
-
 denaturation after 5 min Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 6
-
 D-fructose reduction Gluconobacter oxydans
1.1.1.14 9 10.5 sorbitol oxidation Gluconobacter oxydans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.14 4.5 11 several days at 4°C Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+ specific for Gluconobacter oxydans
1.1.1.14 NADH
-
 Gluconobacter oxydans