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Literature summary extracted from

  • Oura, Y.; Yamada, K.; Shiratake, K.; Yamaki, S.
    Purification and characterization of NAD+-dependent sorbitol dehydrogenase from Japanese pear fruit (2000), Phytochemistry, 54, 567-572.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.14 ZnSO4 reverses cysteine inhibition Pyrus pyrifolia

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.14 cysteine inhibits fructose reduction at pH 9.0, stimulates at pH 7.4 Pyrus pyrifolia
1.1.1.14 HgCl2
-
 Pyrus pyrifolia
1.1.1.14 MgCl2
-
 Pyrus pyrifolia
1.1.1.14 MnSO4
-
 Pyrus pyrifolia

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Pyrus pyrifolia
-
 japanese pear
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.14
-
 Pyrus pyrifolia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.14 fruit
-
 Pyrus pyrifolia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.14 55
-
-
 Pyrus pyrifolia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-ribitol + NAD+
-
 Pyrus pyrifolia ?
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 Pyrus pyrifolia D-fructose + NADH + H+
-
 ?
1.1.1.14 D-xylitol + NAD+
-
 Pyrus pyrifolia ?
-
 ?
1.1.1.14 erythritol + NAD+ low activity Pyrus pyrifolia ?
-
 ?
1.1.1.14 ethanol + NAD+ low activity Pyrus pyrifolia acetaldehyde + NADH
-
 ?
1.1.1.14 L-arabitol + NAD+ low activity Pyrus pyrifolia ?
-
 ?
1.1.1.14 L-iditol + NAD+
-
 Pyrus pyrifolia ?
-
 ?
1.1.1.14 L-threitol + NAD+
-
 Pyrus pyrifolia ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 7
-
 D-fructose reduction Pyrus pyrifolia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.14 5 7.5 less than 20% of maximal activity below pH 5, less than 80% above pH 7.5, fructose reduction Pyrus pyrifolia
1.1.1.14 7.5 9 less than 20% of maximal activity below pH 7.5, 100% at pH 9.0, sorbitol oxidation Pyrus pyrifolia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+
-
 Pyrus pyrifolia
1.1.1.14 NADH
-
 Pyrus pyrifolia