EC Number | General Stability | Organism |
---|---|---|
1.1.1.149 | 20%, v/v, glycerol for stabilization during purification | Tetrahymena pyriformis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.149 | barbiturates | - |
Tetrahymena pyriformis | |
1.1.1.149 | Cu2+ | 40% inhibition | Tetrahymena pyriformis | |
1.1.1.149 | Diphenic acid | less than 17% inhibition | Tetrahymena pyriformis | |
1.1.1.149 | indomethacin | less than 17% inhibition | Tetrahymena pyriformis | |
1.1.1.149 | additional information | no inhibition with thiol reagents, reducing or chelating agents, ADH inhibitors | Tetrahymena pyriformis | |
1.1.1.149 | natural oestrogens | e.g. oestrone less than 15% inhibition | Tetrahymena pyriformis | |
1.1.1.149 | quercitrin | - |
Tetrahymena pyriformis | |
1.1.1.149 | synthetic oestrogens | e.g. hexestrol, silbestrol, dienstrol | Tetrahymena pyriformis | |
1.1.1.149 | valporic acid | less than 17% inhibition | Tetrahymena pyriformis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.149 | 0.0002 | - |
NADPH | 20-oxo and 20alpha-hydroxy-steroids, pH 7.0 | Tetrahymena pyriformis | |
1.1.1.149 | 0.0029 | - |
17alpha-hydroxyprogesterone | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.0034 | - |
17alpha-hydroxypregnenolone | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.008 | - |
NADP+ | 20-oxo and 20alpha-hydroxy-steroids, pH 7.0 | Tetrahymena pyriformis | |
1.1.1.149 | 0.013 | - |
17alpha,20alpha-dihydroxypregn-4-en-3-one | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.02 | - |
17alpha,21-dihydroxy-5beta-pregnane-3,11,20-trione | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.022 | - |
17alpha,21-dihydroxy-5beta-pregnan-3,20-dione | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.064 | - |
isatin | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.067 | - |
ethyl acetoacetate | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.073 | - |
20alpha-hydroxy-5beta-pregnan-3-one | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.078 | - |
Camphorquinone | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.081 | - |
Pyridine-3-aldehyde | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.125 | - |
1,2-Cyclohexanedione | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.21 | - |
2,4-Pentanedione | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.34 | - |
4-nitrobenzaldehyde | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.43 | - |
Phenylglyoxal | - |
Tetrahymena pyriformis | |
1.1.1.149 | 0.67 | - |
D-glyceraldehyde | - |
Tetrahymena pyriformis | |
1.1.1.149 | 2.1 | - |
benzene dihydrodiol | - |
Tetrahymena pyriformis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.149 | 33000 | - |
2 * 33000, SDS-PAGE | Tetrahymena pyriformis |
1.1.1.149 | 68000 | - |
gel filtration | Tetrahymena pyriformis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.149 | additional information | Tetrahymena pyriformis | role of enzyme suggested to be for detoxication or metabolism of carbonyl compounds because of broad substrate specificity | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.149 | Tetrahymena pyriformis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.149 | ammonium sulfate precipitation, ultrafiltration, chromatography | Tetrahymena pyriformis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.149 | 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ | aldoketo reductase superfamily | Tetrahymena pyriformis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.149 | 6.2 | 6.9 | - |
Tetrahymena pyriformis |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.149 | -20°C, purified enzyme, 10 mM Tris/HCl, pH 7.5, 0.5 mM EDTA, 1 mM 2-mercaptoethanol, 20%, v/v, glycerol, 2 weeks, little activity loss | Tetrahymena pyriformis |
1.1.1.149 | 4°C, Sephadex G-100 fraction, rapid decrease, 2 days, 50% activity loss, 80% activity remaining by addition of 20%, v/v, glycerol | Tetrahymena pyriformis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.149 | 1,2-cyclohexanedione + NADPH + H+ | - |
Tetrahymena pyriformis | ? | - |
r | |
1.1.1.149 | 17alpha,21-dihydroxy-5beta-pregnan-3,20-dione + NADPH + H+ | - |
Tetrahymena pyriformis | 17alpha,20alpha,21-trihydroxy-5beta-pregnane-3-one + NADP+ | - |
r | |
1.1.1.149 | 17alpha,21-dihydroxy-5beta-pregnane-3,11,20-trione + NADPH + H+ | - |
Tetrahymena pyriformis | 17alpha,20alpha,21-trihydroxy-5beta-pregnane-3,11-dione + NADP+ | - |
r | |
1.1.1.149 | 17alpha-hydroxypregnenolone + NADPH | - |
Tetrahymena pyriformis | ? + NADP+ | - |
? | |
1.1.1.149 | 17alpha-hydroxyprogesterone + NADPH + H+ | best substrate | Tetrahymena pyriformis | 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NADP+ | - |
r | |
1.1.1.149 | 2,4-pentanedione + NADPH + H+ | - |
Tetrahymena pyriformis | 4-hydroxypentan-2-one + NADP+ | - |
r | |
1.1.1.149 | 20alpha-hydroxy-5beta-pregnan-3-one + NADP+ | - |
Tetrahymena pyriformis | 5beta-pregnane-3,20-dione + NADPH | - |
? | |
1.1.1.149 | 4-nitrobenzaldehyde + NADPH | - |
Tetrahymena pyriformis | 4-nitrobenzyl alcohol + NADP+ | - |
? | |
1.1.1.149 | benzene dihydrodiol + NADP+ | - |
Tetrahymena pyriformis | 2-hydroxycyclohexa-3,5-diene-1-one + NADPH + H+ | - |
r | |
1.1.1.149 | camphorquinone + NADP+ | - |
Tetrahymena pyriformis | ? | - |
r | |
1.1.1.149 | D-glyceraldehyde + NADPH + H+ | - |
Tetrahymena pyriformis | glycerol + NADP+ | - |
r | |
1.1.1.149 | ethyl acetoaetate + NADPH | - |
Tetrahymena pyriformis | (S)-ethyl 3-hydroxybutyrate + NADP+ | - |
? | |
1.1.1.149 | isatin + NADPH | - |
Tetrahymena pyriformis | NADP+ + ? | - |
? | |
1.1.1.149 | additional information | less or no activity with 5beta-androstane-3,17-dione, 17beta-hydroxy-5beta-androstan-3-one, progesterone, 5beta-pregnane-3,20-dione, cortisone oestrone, 20-oxopregnenes without 17alpha-hydroxy group, 20-oxopregnanes, corticosteroides, 3- or 17-oxosteroids, 20beta-hydroxysteroids, 3- or 17-hydroxysteroids, alcohols as substrate, other substrates: isatin, ethyl acetoacetate, camphorquinone, pyridine-3-aldehyde, 1,2-cyclohexanedione, 2,4-pentanedione, 4-nitrobenzaldehyde, benzene-dihydrodiol | Tetrahymena pyriformis | ? | - |
? | |
1.1.1.149 | additional information | role of enzyme suggested to be for detoxication or metabolism of carbonyl compounds because of broad substrate specificity | Tetrahymena pyriformis | ? | - |
? | |
1.1.1.149 | phenylglyoxal + NADPH + H+ | - |
Tetrahymena pyriformis | ? | - |
r | |
1.1.1.149 | pyridine-3-aldehyde + NADPH | - |
Tetrahymena pyriformis | NADP+ + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.149 | dimer | 2 * 33000, SDS-PAGE | Tetrahymena pyriformis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.149 | 25 | - |
10 min at pH 6-10 | Tetrahymena pyriformis |
1.1.1.149 | 40 | - |
up to, for 5 min 95% initial activity, rapidly inactivated above 45°C | Tetrahymena pyriformis |
1.1.1.149 | 45 | - |
rapidly inactivated above | Tetrahymena pyriformis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.149 | 6 | 6.5 | 17alpha-hydroxyprogesterone reduction | Tetrahymena pyriformis |
1.1.1.149 | 10.5 | - |
steroid oxidation | Tetrahymena pyriformis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.149 | 5.8 | 10.5 | 25°C, below and above range inactivation | Tetrahymena pyriformis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.149 | 4.5 | 11.5 | 25°C, below and above range total loss of activity | Tetrahymena pyriformis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.149 | NADP+ | strictly specific | Tetrahymena pyriformis | |
1.1.1.149 | NADPH | strictly specific | Tetrahymena pyriformis |