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Literature summary extracted from

  • Mao, J.; Duan, R.W.; Zhong, L.; Gibori, G.; Azhar, S.
    Expression, purification and characterization of the rat luteal 20alpha-hydroxysteroid dehydrogenase (1997), Endocrinology, 138, 182-190.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.149 expressed in Escherichia coli DH5alpha and baculovirus-insect cell systems Sf9 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.149 0.0058 0.0059 20alpha-hydroxyprogesterone 37°C, 10 min Rattus norvegicus
1.1.1.149 0.0095 0.0096 NADP+ 37°C, 7.5 min Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.149 37000
-
SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.149 progesterone + NADPH Rattus norvegicus central role in luteolysis and parturition 20alpha-hydroxyprogesterone + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.149 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.149 glycoprotein enzyme expressed by insect cells, not essential for catalytic activity Rattus norvegicus
1.1.1.149 phosphoprotein enzyme expressed by insect cells, enzyme expressed by E. coli phosphorylated by various kinases, not essential for catalytic activity Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.149 affinity binding to glutathione-Sepharose and thrombin digestion of bacterial lysate, ion exchange and affinity chromatography of enzyme produced by insect cells Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.149 ovary corpus luteum Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.149 0.2596
-
insect cells Rattus norvegicus
1.1.1.149 0.332
-
+/-0.047, Escherichia coli Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.149 17alpha-hydroxyprogesterone + NADPH + H+
-
Rattus norvegicus 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NADP+
-
?
1.1.1.149 4-nitrobenzaldehyde + NADPH
-
Rattus norvegicus 4-nitrobenzyl alcohol + NADP+
-
?
1.1.1.149 9,10-phenanthrenequinone + NADPH
-
Rattus norvegicus NADP+ + ?
-
?
1.1.1.149 benzaldehyde + NADPH
-
Rattus norvegicus benzyl alcohol + NADP+
-
?
1.1.1.149 D-galactose + NADPH
-
Rattus norvegicus NADP+ + ?
-
?
1.1.1.149 D-glucose + NADPH
-
Rattus norvegicus NADP+ + ?
-
?
1.1.1.149 DL-glyceraldehyde + NADPH
-
Rattus norvegicus glycerol + NADP+
-
?
1.1.1.149 methylglyoxal + NADPH
-
Rattus norvegicus NADP+ + ?
-
?
1.1.1.149 additional information benzaldehyde, DL-glyceraldehyde, 4-nitrobenzaldehyde and glucose very efficient substrates, methylglyoxal, 9,10-phenanthrenquinone and D-galactose less active, no activity with corticosterone and 5alpha-dihydrotestosterone Rattus norvegicus ?
-
?
1.1.1.149 progesterone + NADPH
-
Rattus norvegicus 20alpha-hydroxyprogesterone + NADP+
-
r
1.1.1.149 progesterone + NADPH central role in luteolysis and parturition Rattus norvegicus 20alpha-hydroxyprogesterone + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.149 monomer 1 * 37000, SDS-PAGE Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.149 NADP+
-
Rattus norvegicus
1.1.1.149 NADPH
-
Rattus norvegicus