Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yoon, K.H.; Cha, K.W.; Park, S.I.; Yim, J.J.
    Isolation and characterization of sepiapterin reductase from Drosophila melanogaster (1993), Pteridines, 4, 43-50.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.153 acetic acid buffers that contain acetate inhibit activity Drosophila melanogaster
1.1.1.153 Cd2+ 1 mM inhibits entirely Drosophila melanogaster
1.1.1.153 Fe3+ 1 mM inhibits entirely Drosophila melanogaster
1.1.1.153 additional information Li+, Na+, Co2+, Mn2+, Ni2+ show slight inhibition at 1 mM Drosophila melanogaster
1.1.1.153 NADPH more than 0.05 mM causes substrate inhibition Drosophila melanogaster
1.1.1.153 Sn2+ 1 mM inhibits entirely Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.153 0.0014
-
 NADPH
-
 Drosophila melanogaster
1.1.1.153 0.075
-
 sepiapterin
-
 Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.153 38400
-
 1 * 38400, SDS-PAGE Drosophila melanogaster
1.1.1.153 39000
-
 gel filtration, nondissociating PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH Drosophila melanogaster
-
 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH Drosophila melanogaster Oregon R
-
 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.153 Drosophila melanogaster
-
-
-
1.1.1.153 Drosophila melanogaster Oregon R
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.153
-
 Drosophila melanogaster

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.153 L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+ enzyme also reduces carbonyl compounds of non-pteridine derivatives Drosophila melanogaster
a

Storage Stability

EC Number Storage Stability Organism
1.1.1.153 -40°C, in 50 mM potassium phosphate buffer, pH 6.9, at least stable for two months Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.153 1,2-cyclohexadione + NADPH
-
 Drosophila melanogaster ?
-
 ?
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH
-
 Drosophila melanogaster 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH
-
 Drosophila melanogaster Oregon R 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
1.1.1.153 diacetyl + NADPH
-
 Drosophila melanogaster ?
-
 ?
1.1.1.153 diacetyl + NADPH
-
 Drosophila melanogaster Oregon R ?
-
 ?
1.1.1.153 menadione + NADPH
-
 Drosophila melanogaster ?
-
 ?
1.1.1.153 menadione + NADPH
-
 Drosophila melanogaster Oregon R ?
-
 ?
1.1.1.153 methylglyoxal + NADPH
-
 Drosophila melanogaster ?
-
 ?
1.1.1.153 methylglyoxal + NADPH
-
 Drosophila melanogaster Oregon R ?
-
 ?
1.1.1.153 p-nitrobenzaldehyde + NADPH
-
 Drosophila melanogaster p-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.153 phenylglyoxal + NADPH
-
 Drosophila melanogaster ?
-
 ?
1.1.1.153 phenylglyoxal + NADPH
-
 Drosophila melanogaster Oregon R ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.153 monomer 1 * 38400, SDS-PAGE Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.153 30
-
-
 Drosophila melanogaster

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.153 48
-
 half of activity remains within 30 min Drosophila melanogaster

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.153 5.7 6.7 optimium for activity, highest activity in phosphate buffer Drosophila melanogaster