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Literature summary extracted from

  • Van Laere, A.
    Purification and properties of an NADPH-dependent dihydroxyacetone reductase from Phycomyces spores (1985), FEMS Microbiol. Lett., 30, 377-381.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.156 KCl inhibition above 100 mM Phycomyces blakesleeanus
1.1.1.156 Mg2+ 40% inhibition at 5 mM Phycomyces blakesleeanus
1.1.1.156 Mn2+ 25% inhibition at 5 mM Phycomyces blakesleeanus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.156 0.01
-
 NADPH
-
 Phycomyces blakesleeanus
1.1.1.156 0.167
-
 NADP+
-
 Phycomyces blakesleeanus
1.1.1.156 3.9
-
 dihydroxyacetone
-
 Phycomyces blakesleeanus
1.1.1.156 2437
-
 glycerol
-
 Phycomyces blakesleeanus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.156 34000 39000 gel filtration, SDS-PAGE Phycomyces blakesleeanus
1.1.1.156 39000
-
 1 * 39000, SDS-PAGE Phycomyces blakesleeanus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.156 glycerol + NADP+ Phycomyces blakesleeanus involved in synthesis of glycerol during early spore germination dihydroxyacetone + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.156 Phycomyces blakesleeanus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.156 to homogeneity, chromatography techniques Phycomyces blakesleeanus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.156 sporangiospore
-
 Phycomyces blakesleeanus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.156 31.1
-
-
 Phycomyces blakesleeanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.156 dihydroxyacetonephosphate + NADPH very low activity Phycomyces blakesleeanus glycerol 3-phosphate + NADP+
-
 ?
1.1.1.156 glycerol + NADP+ involved in synthesis of glycerol during early spore germination Phycomyces blakesleeanus dihydroxyacetone + NADPH + H+
-
 r
1.1.1.156 L-arabitol + NADP+ 11% of the activity compared to glycerol Phycomyces blakesleeanus ? + NADPH
-
 ?
1.1.1.156 L-glyceraldehyde + NADPH very low activity Phycomyces blakesleeanus L-glycerol + NADP+
-
 ?
1.1.1.156 meso-erythritol + NADP+ 35% of the activity compared to glycerol Phycomyces blakesleeanus ? + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.156 monomer 1 * 39000, SDS-PAGE Phycomyces blakesleeanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.156 6 6.5 dihydroxyacetone reduction Phycomyces blakesleeanus
1.1.1.156 9.4
-
 glycerol oxidation Phycomyces blakesleeanus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.156 NADP+ can not be replaced by NAD+ Phycomyces blakesleeanus