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Literature summary extracted from

  • Taku, A.; Gunetileke, K.G.; Anwar, R.A.
    Biosynthesis of uridine diphospho-N-acetylmuramic acid. 3. Purification and properties of uridine diphospho-N-acetylenolpyruvyl-glucosamine reductase (1970), J. Biol. Chem., 245, 5012-5016.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.98 FAD 11-25% stimulation Enterobacter cloacae

General Stability

EC Number General Stability Organism
1.3.1.98 dithiothreitol stabilizes Enterobacter cloacae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.98 iodoacetamide
-
Enterobacter cloacae
1.3.1.98 N-ethylmaleimide
-
Enterobacter cloacae
1.3.1.98 p-chloromercuribenzoate
-
Enterobacter cloacae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.98 0.04
-
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
Enterobacter cloacae
1.3.1.98 0.049
-
NADPH
-
Enterobacter cloacae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Enterobacter cloacae
-
UDP-N-acetyl-3-D-muramate + NADP+
-
?
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Enterobacter cloacae NRC 492
-
UDP-N-acetyl-3-D-muramate + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.98 Enterobacter cloacae
-
NRC 492
-
1.3.1.98 Enterobacter cloacae NRC 492
-
NRC 492
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.3.1.98 very sensitive to oxidation, rapid loss of activity in absence of added dithiothreitol Enterobacter cloacae

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.98
-
Enterobacter cloacae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.98 0.015
-
-
Enterobacter cloacae

Storage Stability

EC Number Storage Stability Organism
1.3.1.98 -20°C, pH 8.0, presence of dithiothreitol, up to 2 months without loss of activity Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
Enterobacter cloacae UDP-N-acetyl-3-D-muramate + NADP+
-
?
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
Enterobacter cloacae NRC 492 UDP-N-acetyl-3-D-muramate + NADP+
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.98 40
-
increase of activity up to 40°C Enterobacter cloacae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.1.98 40 50 40°C: activity maximum, 50°C: about 30% of activity maximum Enterobacter cloacae
1.3.1.98 50
-
at 50°C about 30% of maximal activity Enterobacter cloacae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.98 8.1 8.5 in Tris-HCl buffer maximal activity Enterobacter cloacae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.98 7.1 9.9 at pH 7.1 and pH 9.9 about 60% of maximal activity Enterobacter cloacae

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.98 FAD prosthetic group: FAD, Na2S2O4, NaBH4 and to a lesser extent NADH can replace NADPH Enterobacter cloacae
1.3.1.98 additional information no effect with FMN Enterobacter cloacae
1.3.1.98 NADH reductase activity with NADH is only 5% of that with NADPH Enterobacter cloacae
1.3.1.98 NADPH
-
Enterobacter cloacae