EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.98 | FAD | 11-25% stimulation | Enterobacter cloacae |
EC Number | General Stability | Organism |
---|---|---|
1.3.1.98 | dithiothreitol stabilizes | Enterobacter cloacae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.98 | iodoacetamide | - |
Enterobacter cloacae | |
1.3.1.98 | N-ethylmaleimide | - |
Enterobacter cloacae | |
1.3.1.98 | p-chloromercuribenzoate | - |
Enterobacter cloacae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.98 | 0.04 | - |
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine | - |
Enterobacter cloacae | |
1.3.1.98 | 0.049 | - |
NADPH | - |
Enterobacter cloacae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.98 | UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | Enterobacter cloacae | - |
UDP-N-acetyl-3-D-muramate + NADP+ | - |
? | |
1.3.1.98 | UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | Enterobacter cloacae NRC 492 | - |
UDP-N-acetyl-3-D-muramate + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.98 | Enterobacter cloacae | - |
NRC 492 | - |
1.3.1.98 | Enterobacter cloacae NRC 492 | - |
NRC 492 | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.3.1.98 | very sensitive to oxidation, rapid loss of activity in absence of added dithiothreitol | Enterobacter cloacae |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.98 | - |
Enterobacter cloacae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.98 | 0.015 | - |
- |
Enterobacter cloacae |
EC Number | Storage Stability | Organism |
---|---|---|
1.3.1.98 | -20°C, pH 8.0, presence of dithiothreitol, up to 2 months without loss of activity | Enterobacter cloacae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.98 | UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | - |
Enterobacter cloacae | UDP-N-acetyl-3-D-muramate + NADP+ | - |
? | |
1.3.1.98 | UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | - |
Enterobacter cloacae NRC 492 | UDP-N-acetyl-3-D-muramate + NADP+ | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.1.98 | 40 | - |
increase of activity up to 40°C | Enterobacter cloacae |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.1.98 | 40 | 50 | 40°C: activity maximum, 50°C: about 30% of activity maximum | Enterobacter cloacae |
1.3.1.98 | 50 | - |
at 50°C about 30% of maximal activity | Enterobacter cloacae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.98 | 8.1 | 8.5 | in Tris-HCl buffer maximal activity | Enterobacter cloacae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.98 | 7.1 | 9.9 | at pH 7.1 and pH 9.9 about 60% of maximal activity | Enterobacter cloacae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.98 | FAD | prosthetic group: FAD, Na2S2O4, NaBH4 and to a lesser extent NADH can replace NADPH | Enterobacter cloacae | |
1.3.1.98 | additional information | no effect with FMN | Enterobacter cloacae | |
1.3.1.98 | NADH | reductase activity with NADH is only 5% of that with NADPH | Enterobacter cloacae | |
1.3.1.98 | NADPH | - |
Enterobacter cloacae |