Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dahms, A.S.; Russo, J.
    D-Xylose dehydrogenase (1982), Methods Enzymol., 89, 226-228.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.175 thermolabile, stabilizing: substrates or high ionic strength Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.175 0.2
-
 NAD+
-
 Pseudomonas sp.
1.1.1.175 0.5
-
 D-xylose
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.175 D-xylose + NAD+ Pseudomonas sp. highly specific for both substrates D-xylonolactone + NADH + H+
-
 ?
1.1.1.175 D-xylose + NAD+ Pseudomonas sp. MSU-1 highly specific for both substrates D-xylonolactone + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.175 Pseudomonas sp.
-
-
-
1.1.1.175 Pseudomonas sp. MSU-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.175 NAD+-affinity chromatography Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.175 culture condition:D-xylose-grown cell inducible enzyme synthesis by growth in D-xylose-containing medium Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.175 60.3
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.175 frozen, addition of DTT, several weeks Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.175 D-xylose + NAD+ highly specific for both substrates Pseudomonas sp. D-xylonolactone + NADH + H+
-
 ?
1.1.1.175 D-xylose + NAD+ highly specific for both substrates Pseudomonas sp. MSU-1 D-xylonolactone + NADH + H+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.175 32
-
 half-life: 2 min Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.175 8
-
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.175 NAD+ cannot be replaced by NADP+ Pseudomonas sp.