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Literature summary extracted from

  • Bernardo, A.; Burgos, J.; Martin, R.
    Purification and some properties of L-glycol dehydrogenase from hens muscle (1981), Biochim. Biophys. Acta, 659, 189-198.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.185 easily inactivated in high molarity buffers Gallus gallus
1.1.1.185 very stable in both water and low molarity buffers of pH 7 at 0-4°C Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.185 additional information
-
 additional information
-
 Gallus gallus
1.1.1.185 0.026
-
 glyceraldehyde pI 7.2 form Gallus gallus
1.1.1.185 0.026
-
 glyceraldehyde enzyme form of pI 7.2, at pH 7.0 and 25°C Gallus gallus
1.1.1.185 0.029
-
 glyceraldehyde pI 6.2 form Gallus gallus
1.1.1.185 0.029
-
 glyceraldehyde enzyme form of pI 6.2, at pH 7.0 and 25°C Gallus gallus
1.1.1.185 0.053
-
 Glyoxal pI 6.2 form Gallus gallus
1.1.1.185 0.053
-
 Glyoxal enzyme form of pI 6.2, at pH 7.0 and 25°C Gallus gallus
1.1.1.185 0.06
-
 2,3-Pentanedione pI 7.2 form Gallus gallus
1.1.1.185 0.097
-
 2,3-Pentanedione pI 6.2 form Gallus gallus
1.1.1.185 0.098
-
 methylglyoxal pI 6.2 form Gallus gallus
1.1.1.185 0.102
-
 methylglyoxal pI 7.2 form Gallus gallus
1.1.1.185 0.109
-
 diacetyl pI 7.2 form Gallus gallus
1.1.1.185 0.38
-
 diacetyl pI 6.2 form Gallus gallus
1.1.1.185 0.411
-
 Glyoxal pI 4.8 form Gallus gallus
1.1.1.185 0.411
-
 Glyoxal enzyme form of pI 4.8, at pH 7.0 and 25°C Gallus gallus
1.1.1.185 0.522
-
 Glyoxal pI 7.2 form Gallus gallus
1.1.1.185 0.522
-
 Glyoxal enzyme form of pI 7.2, at pH 7.0 and 25°C Gallus gallus
1.1.1.185 0.566
-
 glyceraldehyde pI 4.8 form Gallus gallus
1.1.1.185 0.566
-
 glyceraldehyde enzyme form of pI 4.8, at pH 7.0 and 25°C Gallus gallus
1.1.1.185 0.675
-
 2,3-Pentanedione pI 4.8 form Gallus gallus
1.1.1.185 2.013
-
 acetoin pI 6.2 form Gallus gallus
1.1.1.185 2.164
-
 acetoin pI 4.8 form Gallus gallus
1.1.1.185 2.59
-
 acetoin pI 7.2 form Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.185 28000
-
 gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.185 Gallus gallus
-
 hen
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.185 3 enzyme forms: pI 7.2, pI 6.2 and pI 4.8 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.185 muscle
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.185 additional information
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
1.1.1.185 -18°C, enzyme at any stage of purification Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.185 2,3-pentanedione + NADH
-
 Gallus gallus L-acetylethylcarbinol + NAD+
-
 ?
1.1.1.185 diacetyl + NADH
-
 Gallus gallus L-acetoin + NAD+
-
 ?
1.1.1.185 ethyl pyruvate + NAD(P)H
-
 Gallus gallus ethyl lactate
-
 ?
1.1.1.185 glyceraldehyde + NADPH + H+
-
 Gallus gallus ?
-
 r
1.1.1.185 glyoxal + NADPH + H+
-
 Gallus gallus ?
-
 r
1.1.1.185 methyl pyruvate + NAD(P)H
-
 Gallus gallus ?
-
 ?
1.1.1.185 methylglyoxal + NADH
-
 Gallus gallus L-lactaldehyde
-
 ?
1.1.1.185 additional information not: acetaldehyde, monoketones, non-vicinal diketones, free ketoacids Gallus gallus ?
-
 ?
1.1.1.185 R1-CO-CHOH-R2 + NAD(P)H 2-hydroxycarbonyl compound e.g. glycolaldehyde, glyceraldehyde, acetoin, acetylethylcarbinol, diacetylmethylcarbinol Gallus gallus R1-CHOH-CHOH-R2 + NAD(P)+
-
 r
1.1.1.185 R1-CO-CHOH-R2 + NAD(P)H much more efficient in dehydrogenase direction Gallus gallus R1-CHOH-CHOH-R2 + NAD(P)+
-
 r
1.1.1.185 R1-CO-CO-R2 + NAD(P)H methylglyoxal, diacetyl, 2,3-pentanedione Gallus gallus R1-CO-CHOH-R2 + NAD(P)+
-
 ?
1.1.1.185 R1-CO-CO-R2 + NAD(P)H vicinal dicarbonyl: e.g. glyoxal Gallus gallus R1-CO-CHOH-R2 + NAD(P)+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.185 25
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.185 5 6.6
-
 Gallus gallus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.185 7
-
 very stable in both water and low molarity buffers of pH 7 at 0-4°C Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.185 additional information no activity with alpha-NADH Gallus gallus
1.1.1.185 NAD+
-
 Gallus gallus
1.1.1.185 NADH
-
 Gallus gallus
1.1.1.185 NADPH
-
 Gallus gallus