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Literature summary extracted from

  • Das, B.; Srivastava, S.K.
    Purification and properties of aldose reductase and aldehyde reductase II from human erythrocyte (1985), Arch. Biochem. Biophys., 238, 670-679.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.21 freezing of purified enzyme results in complete loss of activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.2 (NH4)2SO4
-
 Homo sapiens
1.1.1.2 3,3'-tetramethylenglutaric acid
-
 Homo sapiens
1.1.1.2 Alrestatin
-
 Homo sapiens
1.1.1.2 Li2SO4 0.4 mM completely inhibits aldehyde reductase II Homo sapiens
1.1.1.2 N-ethylmaleimide
-
 Homo sapiens
1.1.1.2 p-hydroxymercuribenzoate
-
 Homo sapiens
1.1.1.2 quercetrin
-
 Homo sapiens
1.1.1.2 sodium phenobarbital
-
 Homo sapiens
1.1.1.2 sorbinil
-
 Homo sapiens
1.1.1.21 (S)-6-fluorospiro[chroman-4,5'-imidazolidine]-2',4'-dione
-
 Homo sapiens
1.1.1.21 3,3-Tetramethylene glutaric acid
-
 Homo sapiens
1.1.1.21 Alrestatin 1,3-dioxo-1H-benz[de]isoquinoline-2-(3H)acetic acid Homo sapiens
1.1.1.21 Li2SO4 0.4 M: essential for full activity Homo sapiens
1.1.1.21 N-ethylmaleimide
-
 Homo sapiens
1.1.1.21 p-hydroxymercuribenzoate
-
 Homo sapiens
1.1.1.21 Phenobarbital
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.019
-
 NADPH aldehyde reductase II Homo sapiens
1.1.1.2 0.29
-
 3-Pyridinecarboxaldehyde aldehyde reductase II Homo sapiens
1.1.1.2 2.5
-
 glucuronate aldehyde reductase II Homo sapiens
1.1.1.2 7.59
-
 glyceraldehyde aldehyde reductase II Homo sapiens
1.1.1.2 14.54
-
 propionaldehyde aldehyde reductase II Homo sapiens
1.1.1.21 0.01 0.015 NADPH
-
 Homo sapiens
1.1.1.21 0.011
-
 propionaldehyde
-
 Homo sapiens
1.1.1.21 0.039
-
 Pyridine-3-aldehyde
-
 Homo sapiens
1.1.1.21 0.51
-
 glyceraldehyde
-
 Homo sapiens
1.1.1.21 0.62
-
 NADH
-
 Homo sapiens
1.1.1.21 1.18
-
 D-glucuronate
-
 Homo sapiens
1.1.1.21 102
-
 ribose
-
 Homo sapiens
1.1.1.21 142
-
 galactose
-
 Homo sapiens
1.1.1.21 155
-
 glucose
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.21 Li2SO4 0.4 M: essential for full expression of enzyme activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 32500
-
 gel filtration, Sephadex G-100 Homo sapiens
1.1.1.21 32500
-
 1 * 32500, SDS-PAGE in presence of urea and 2-mercaptoethanol Homo sapiens
1.1.1.21 34300
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.2 D-glucuronate + NADPH Homo sapiens
-
 L-gulonate + NADP+
-
 r
1.1.1.2 D-glyceraldehyde + NADPH + H+ Homo sapiens
-
 glycerol + NADP+
-
 r
1.1.1.2 L-glyceraldehyde + NADPH Homo sapiens
-
 glycerol + NADP+
-
 r
1.1.1.21 aldose + NAD(P)H Homo sapiens
-
 alditol + NAD(P)+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Homo sapiens
-
 human
-
1.1.1.21 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2 aldehyde reductase II Homo sapiens
1.1.1.21
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.2 brain
-
 Homo sapiens
-
1.1.1.2 erythrocyte
-
 Homo sapiens
-
1.1.1.2 liver
-
 Homo sapiens
-
1.1.1.2 placenta
-
 Homo sapiens
-
1.1.1.21 erythrocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 3-pyridinecarboxaldehyde + NADPH
-
 Homo sapiens 3-pyridinemethanol + NADP+
-
 r
1.1.1.2 D-glucuronate + NADPH
-
 Homo sapiens L-gulonate + NADP+
-
 ?
1.1.1.2 D-glucuronate + NADPH
-
 Homo sapiens L-gulonate + NADP+
-
 r
1.1.1.2 D-glyceraldehyde + NADPH + H+
-
 Homo sapiens glycerol + NADP+
-
 r
1.1.1.2 glyceraldehyde + NADPH + H+
-
 Homo sapiens glycerol + NADP+
-
 r
1.1.1.2 L-glyceraldehyde + NADPH
-
 Homo sapiens glycerol + NADP+
-
 r
1.1.1.2 additional information enzyme is virtually inactive for aldo-sugars Homo sapiens ?
-
 ?
1.1.1.2 propionaldehyde + NADPH
-
 Homo sapiens propanol + NADP+
-
 r
1.1.1.21 2-D-deoxygalactose + NADPH
-
 Homo sapiens ?
-
 ?
1.1.1.21 alditol + NAD(P)+
-
 Homo sapiens aldose + NAD(P)H
-
 r
1.1.1.21 aldose + NAD(P)H
-
 Homo sapiens alditol + NAD(P)+
-
 r
1.1.1.21 D-galactose + NAD(P)H
-
 Homo sapiens dulcitol + NAD(P)+
-
 r
1.1.1.21 D-glucose + NAD(P)H
-
 Homo sapiens sorbitol + NAD(P)+
-
 r
1.1.1.21 D-glucuronate + NAD(P)H
-
 Homo sapiens ?
-
 r
1.1.1.21 D-ribose + NAD(P)H
-
 Homo sapiens ribitol + NAD(P)+
-
 r
1.1.1.21 propionaldehyde + NAD(P)H
-
 Homo sapiens propanol + NAD(P)+
-
 ?
1.1.1.21 pyridine-3-aldehyde + NADPH + H+
-
 Homo sapiens 3-pyridinemethanol + NADP+
-
 r
1.1.1.21 pyridinecarboxaldehyde + NAD(P)H 2-, 3- and 4-pyridinecarboxaldehyde Homo sapiens pyridylmethanol + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.2 monomer 1 * 32500, SDS-PAGE Homo sapiens
1.1.1.21 monomer 1 * 32500, SDS-PAGE in presence of urea and 2-mercaptoethanol Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.2 L-hexonate dehydrogenase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 35
-
 aldehyde reductase II Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 50
-
 completely inactivated between 5 min Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 7
-
 aldehyde reductase II Homo sapiens
1.1.1.21 6.2
-
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADPH aldehyde reductase II utilizes only NADPH as cofactor Homo sapiens
1.1.1.21 NADH
-
 Homo sapiens