EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | hexadecane | induces enzyme | Acinetobacter sp. |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.2 | synthesis | develpoment of conversion processes for petrochemicals and oil-contaminated environments, cinnamyl aldehyde and cinnamyl alcohol used in flavor and perfume industry, anisaldehyde is used for perfume and toilet soaps, decylalcohol is used in the manufacture of plasticizers, a production system for this enzyme may be useful for industrial application as a biocatalyst in the future | Acinetobacter sp. |
1.1.1.2 | synthesis | reduction of industrially important compounds cinnamyl aldehyde and anisaldehyde, industrial bioconversion of useful alcohols and aldehydes | Acinetobacter sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.2 | alrA gene PCR amplified, cloned and highly expressed in Escherichia coli JM109 | Acinetobacter sp. |
EC Number | General Stability | Organism |
---|---|---|
1.1.1.2 | unstable | Acinetobacter calcoaceticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | Ag+ | 100% inhibition | Acinetobacter sp. | |
1.1.1.2 | Ca2+ | 26% inhibition | Acinetobacter sp. | |
1.1.1.2 | Co2+ | 91% inhibition | Acinetobacter sp. | |
1.1.1.2 | Hg2+ | 100% inhibition | Acinetobacter sp. | |
1.1.1.2 | iodoacetamide | - |
Acinetobacter sp. | |
1.1.1.2 | Mn2+ | 29% inhibition | Acinetobacter sp. | |
1.1.1.2 | N-ethylmaleimide | - |
Acinetobacter sp. | |
1.1.1.2 | Ni2+ | 84% inhibition | Acinetobacter sp. | |
1.1.1.2 | p-mercuribenzoate | - |
Acinetobacter sp. | |
1.1.1.2 | Zn2+ | 100% inhibition | Acinetobacter sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 40000 | - |
4 * 40000, SDS-PAGE | Acinetobacter sp. |
1.1.1.2 | 160000 | - |
gel filtration | Acinetobacter sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.2 | Acinetobacter calcoaceticus | - |
strainNCIB8250, strain HO1-N | - |
1.1.1.2 | Acinetobacter sp. | - |
- |
- |
1.1.1.2 | Acinetobacter sp. M-1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.2 | - |
Acinetobacter calcoaceticus |
1.1.1.2 | wild-type and recombinant protein | Acinetobacter sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 1.7 | - |
recombinant enzyme, expressed in Escherichia coli JM109 | Acinetobacter sp. |
1.1.1.2 | 560 | - |
- |
Acinetobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.2 | 2,4-hexadienal + NADPH | - |
Acinetobacter sp. | 2,4-hexadien-1-ol + NADP+ | - |
? | |
1.1.1.2 | 2-decanone + NADPH | - |
Acinetobacter sp. | 2-decanol + NADP+ | - |
? | |
1.1.1.2 | 2-methylundecanal + NADPH | - |
Acinetobacter sp. | 2-methylundecanol + NADP+ | - |
? | |
1.1.1.2 | 3-heptanone + NADPH | - |
Acinetobacter sp. | 3-heptanol + NADP+ | - |
? | |
1.1.1.2 | 3-octanone + NADPH | - |
Acinetobacter sp. | 3-octanol + NADP+ | - |
? | |
1.1.1.2 | acetaldehyde + NADPH + H+ | - |
Acinetobacter sp. | ethanol + NADP+ | - |
? | |
1.1.1.2 | acetaldehyde + NADPH + H+ | - |
Acinetobacter sp. M-1 | ethanol + NADP+ | - |
? | |
1.1.1.2 | anisaldehyde + NADPH + H+ | - |
Acinetobacter sp. | anisyl alcohol + NADP+ | - |
r | |
1.1.1.2 | benzaldehyde + NADPH | - |
Acinetobacter sp. | benzyl alcohol + NADP+ | - |
r | |
1.1.1.2 | benzaldehyde + NADPH | - |
Acinetobacter sp. M-1 | benzyl alcohol + NADP+ | - |
r | |
1.1.1.2 | hexadecanol + NADP+ | - |
Acinetobacter calcoaceticus | hexadecanal + NADPH | - |
r | |
1.1.1.2 | hexanal + NADPH + H+ | - |
Acinetobacter sp. | hexanol + NADP+ | - |
r | |
1.1.1.2 | hexanal + NADPH + H+ | - |
Acinetobacter sp. M-1 | hexanol + NADP+ | - |
r | |
1.1.1.2 | m-fluorobenzaldehyde + NADPH + H+ | - |
Acinetobacter sp. | m-fluorobenzyl alcohol + NADP+ | - |
? | |
1.1.1.2 | m-methylbenzaldehyde + NADPH | - |
Acinetobacter sp. | m-methylbenzyl alcohol + NADP+ | - |
? | |
1.1.1.2 | n-butyraldehyde + NADPH | - |
Acinetobacter sp. | n-butanol + NADP+ | - |
r | |
1.1.1.2 | n-decanal + NADPH + H+ | - |
Acinetobacter sp. | n-decanol + NADP+ | - |
r | |
1.1.1.2 | n-dodecanal + NADPH + H+ | - |
Acinetobacter sp. | n-dodecanol + NADP+ | - |
r | |
1.1.1.2 | n-heptanal + NADPH | - |
Acinetobacter sp. | heptanol + NADP+ | - |
r | |
1.1.1.2 | n-octanal + NADPH + H+ | - |
Acinetobacter sp. | n-octanol + NADP+ | - |
r | |
1.1.1.2 | nonanal + NADPH | - |
Acinetobacter sp. | ? + NADP+ | - |
? | |
1.1.1.2 | o-fluorobenzaldehyde + NADPH + H+ | - |
Acinetobacter sp. | o-fluorobenzyl alcohol + NADP+ | - |
? | |
1.1.1.2 | o-methylbenzaldehyde + NADPH | - |
Acinetobacter sp. | o-methylbenzyl alcohol + NADP+ | - |
? | |
1.1.1.2 | p-fluorobenzaldehyde + NADPH + H+ | - |
Acinetobacter sp. | p-fluorobenzyl alcohol + NADP+ | - |
? | |
1.1.1.2 | p-methylbenzaldehyde + NADPH | - |
Acinetobacter sp. | p-methylbenzyl alcohol + NADP+ | - |
? | |
1.1.1.2 | pentanal + NADPH | - |
Acinetobacter sp. | pentanol + NADP+ | - |
r | |
1.1.1.2 | pentanal + NADPH | - |
Acinetobacter sp. M-1 | pentanol + NADP+ | - |
r | |
1.1.1.2 | phenylacetaldehyde + NADPH + H+ | - |
Acinetobacter sp. | phenylethanol + NADP+ | - |
r | |
1.1.1.2 | phenylacetaldehyde + NADPH + H+ | - |
Acinetobacter sp. M-1 | phenylethanol + NADP+ | - |
r | |
1.1.1.2 | propanal + NADPH + H+ | - |
Acinetobacter sp. | propanol + NADP+ | - |
r | |
1.1.1.2 | tetradecanal + NADPH | - |
Acinetobacter sp. | tetradecanol | - |
r | |
1.1.1.2 | trans-2-decenal + NADPH | - |
Acinetobacter sp. | trans-2-decen-1-ol + NADP+ | - |
? | |
1.1.1.2 | trans-cinnamaldehyde + NADPH | - |
Acinetobacter sp. | cinnamyl alcohol + NADP+ | - |
? | |
1.1.1.2 | tridecanal + NADPH | - |
Acinetobacter sp. | tridecanol + NADP+ | - |
r | |
1.1.1.2 | undecanal + NADPH | - |
Acinetobacter sp. | undecanol + NADP+ | - |
? | |
1.1.1.2 | vitamin A aldehyde + NADPH | - |
Acinetobacter sp. | ? + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.2 | tetramer | 4 * 40000, SDS-PAGE | Acinetobacter sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 70 | - |
retains 90% activity after 40 min | Acinetobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 7 | - |
n-heptanal reduction | Acinetobacter sp. |
1.1.1.2 | 9.5 | - |
n-heptanol oxidation | Acinetobacter sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 8 | 9 | - |
Acinetobacter sp. |