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Literature summary extracted from

  • Ryle, C.M.; Dowling, T.G.; Tipton, K.F.
    Purification and properties of low-KM aldehyde reductase from ox brain (1984), Biochim. Biophys. Acta, 791, 155-163.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.21 Sodium phosphate at pH 5.0-5.8 and pH 7.2 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.21 Cl- at high ionic strength Bos taurus
1.1.1.21 NaCl
-
 Bos taurus
1.1.1.21 NaNO3
-
 Bos taurus
1.1.1.21 p-Carboxybenzaldehyde substrate inhibition at higher concentration Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.21 0.285
-
 Succinic semialdehyde
-
 Bos taurus
1.1.1.21 1.58
-
 D-glucuronate
-
 Bos taurus
1.1.1.21 1.67
-
 xylose
-
 Bos taurus
1.1.1.21 113
-
 D-glucose 6-phosphate
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.21 31000
-
 gel filtration Bos taurus
1.1.1.21 35100
-
 1 * 35100, SDS-PAGE in presence of 2-mercaptoethanol Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.21 aldose + NAD(P)H Bos taurus
-
 alditol + NAD(P)+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.21 Bos taurus
-
 ox, low-KM enzyme subtype
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.21 ox Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.21 brain ox Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.21 333.3
-
 purified enzyme, with NADH in sodium phosphate buffer 100 mM, pH 7.0 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.21 alditol + NAD(P)+
-
 Bos taurus aldose + NAD(P)H
-
 r
1.1.1.21 aldose + NAD(P)H
-
 Bos taurus alditol + NAD(P)+
-
 r
1.1.1.21 D-glucose 6-phosphate + NADPH + H+
-
 Bos taurus ?
-
 r
1.1.1.21 D-glucuronate + NAD(P)H
-
 Bos taurus ?
-
 r
1.1.1.21 D-xylose + NAD(P)H
-
 Bos taurus xylitol + NAD(P)+
-
 r
1.1.1.21 pyridine-3-aldehyde + NAD(P)H low-KM enzyme subtype Bos taurus 3-hydroxypyridine + NAD(P)+
-
 r
1.1.1.21 succinic semialdehyde + NADH + H+
-
 Bos taurus 4-hydroxybutyrate + NAD+
-
 ?
1.1.1.21 xylitol + NAD(P)+
-
 Bos taurus D-xylose + NAD(P)H + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.21 monomer 1 * 35100, SDS-PAGE in presence of 2-mercaptoethanol Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.21 6.2
-
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.21 NADH
-
 Bos taurus
1.1.1.21 NADP+
-
 Bos taurus