Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bohren, K.M.; Grimshaw, C.E.; Lai, C.J.; Harrison, D.H.; Ringe, D.; Petsko, G.A.; Gabbay, K.H.
    Tyrosine-48 is the proton donor and histidine-110 directs substrate stereochemical selectivity in the reduction reaction of human aldose reductase: enzyme kinetics and crystal structure of the Y48H mutant enzyme (1994), Biochemistry, 33, 2021-2032.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.21 overexpression Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.21
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.21 H110A mutant enzyme Homo sapiens
1.1.1.21 H110Q mutant enzyme Homo sapiens
1.1.1.21 K77M mutant enzyme Homo sapiens
1.1.1.21 Y48F mutant enzyme Homo sapiens
1.1.1.21 Y48H changing substrate selectivity Homo sapiens
1.1.1.21 Y48S mutant enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.21 citrate inhibition is pH-dependent Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.21 0.02
-
 DL-glyceraldehyde
-
 Homo sapiens
1.1.1.21 7.4
-
 D-xylose
-
 Homo sapiens
1.1.1.21 12
-
 DL-glyceraldehyde mutant H110Q Homo sapiens
1.1.1.21 64
-
 DL-glyceraldehyde mutant Y48S Homo sapiens
1.1.1.21 71
-
 DL-glyceraldehyde mutant Y48H Homo sapiens
1.1.1.21 84
-
 DL-glyceraldehyde mutant H110A Homo sapiens
1.1.1.21 111
-
 L-Xylose
-
 Homo sapiens
1.1.1.21 360
-
 L-Xylose mutant H110A Homo sapiens
1.1.1.21 506
-
 D-Lyxose mutant H110Q Homo sapiens
1.1.1.21 861
-
 D-xylose mutant H110Q Homo sapiens
1.1.1.21 1190
-
 L-Xylose mutant H110Q Homo sapiens
1.1.1.21 1340
-
 D-xylose mutant H110A Homo sapiens
1.1.1.21 2630
-
 D-Lyxose
-
 Homo sapiens
1.1.1.21 2740
-
 D-Lyxose mutant H110A Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.21 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.21 alditol + NAD(P)+ = aldose + NAD(P)H + H+ reaction mechanism Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.21 4-nitrobenzaldehyde + NAD(P)H
-
 Homo sapiens 4-nitrobenzyl alcohol + NAD(P)+
-
 ?
1.1.1.21 D-lyxose + NADPH
-
 Homo sapiens arabitol + NADP+
-
 ?
1.1.1.21 DL-glyceraldehyde + NAD(P)H
-
 Homo sapiens glycerol + NAD(P)+
-
 r
1.1.1.21 L-xylose + NAD(P)H
-
 Homo sapiens L-xylitol + NAD(P)+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.21 additional information
-
 DL-glyceraldehyde turnover number in mol per seconds for several mutants Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.21 NADPH NADPH-specific, pro-4R hydrogen is transferred from the nicotinamide ring of the coenzyme to the substrate Homo sapiens