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Literature summary extracted from

  • Adachi, O.; Chiyonobu, T.; Shinagawa, E.; Matsushita, K.; Ameyama, M.
    Crystalline 2-ketogluconate reductase from Acetobacter ascendens, the second instance of crystalline enzyme in genus Acetobacter (1978), Agric. Biol. Chem., 42, 2057-2062.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.215
-
 Acetobacter ascendens

General Stability

EC Number General Stability Organism
1.1.1.215 2-ketogluconate enhances thermal and storage stability even of dilute enzyme solutions, 0.1 mg protein/l Acetobacter ascendens
1.1.1.215 5-ketogluconate does not stabilize Acetobacter ascendens
1.1.1.215 gluconate enhances thermal and storage stability even of dilute enzyme solutions, 0.1 mg protein/l Acetobacter ascendens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.215 Hg2+ complete inhibition Acetobacter ascendens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.215 0.0074
-
 NADPH
-
 Acetobacter ascendens
1.1.1.215 1.2
-
 NADP+
-
 Acetobacter ascendens
1.1.1.215 4.4
-
 gluconate
-
 Acetobacter ascendens
1.1.1.215 5.3
-
 2-keto-D-gluconate
-
 Acetobacter ascendens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.215 cytoplasm
-
 Acetobacter ascendens 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.215 additional information no metal requirement Acetobacter ascendens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.215 40000
-
 3 * 40000, SDS-PAGE Acetobacter ascendens
1.1.1.215 120000
-
 gel filtration Acetobacter ascendens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.215 Acetobacter ascendens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.215 affinity chromatography on Blue-Dextran Sepharose B Acetobacter ascendens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.215 187
-
-
 Acetobacter ascendens

Storage Stability

EC Number Storage Stability Organism
1.1.1.215 ammonium sulfate solution of crystalline enzyme stable for over 2 years Acetobacter ascendens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.215 2-dehydro-D-gluconate + NADPH i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter Acetobacter ascendens D-gluconate + NADP+
-
 r
1.1.1.215 2-dehydro-D-gluconate + NADPH equal reaction rate of reduction of 2-ketogluconate and oxidation of gluconate with Gluconobacter enzyme Acetobacter ascendens D-gluconate + NADP+
-
 r
1.1.1.215 2-keto-L-gulonate + NADPH
-
 Acetobacter ascendens L-idonate + NADP+
-
 r
1.1.1.215 5-keto-D-gluconate + NADPH poor substrate Acetobacter ascendens D-gluconate + NADP+
-
 ?
1.1.1.215 D-galactonate + NADP+
-
 Acetobacter ascendens 2-dehydro-D-galactonate + NADPH
-
 r
1.1.1.215 L-idonate + NADP+
-
 Acetobacter ascendens 2-keto-L-idonate + NADPH
-
 ?
1.1.1.215 additional information D-xylonate, D-glucose, D-fructose, L-sorbose, 5-keto-D-fructose, D-sorbitol, glycerol Acetobacter ascendens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.215 trimer 3 * 40000, SDS-PAGE Acetobacter ascendens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.215 55
-
-
 Acetobacter ascendens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.215 35 65 about half-maximal activity at 35°C and 65°C Acetobacter ascendens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.215 42
-
 stable for 10 min, oxidation activity Acetobacter ascendens
1.1.1.215 53
-
 stable for 10 min, reduction activity Acetobacter ascendens
1.1.1.215 70
-
 stable for 10 min in the presence of either 2-ketogluconate or gluconate Acetobacter ascendens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.215 6
-
 reduction of 2-ketogluconate Acetobacter ascendens
1.1.1.215 10.5
-
-
 Acetobacter ascendens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.215 4.5 8.5 about half-maximal activity at pH 4.5 and 8.5, reduction of ketogluconate Acetobacter ascendens
1.1.1.215 10 12 about half-maximal activity at pH 10 and 12, oxidation of gluconate Acetobacter ascendens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.215 NADP+
-
 Acetobacter ascendens
1.1.1.215 NADPH
-
 Acetobacter ascendens