EC Number | General Stability | Organism |
---|---|---|
1.14.13.51 | 5-10 vol% ethanol stabilizes the crude cell extract | Rhodococcus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.241 | 6-endo-hydroxycineole + NAD(P)+ | Rhodococcus sp. | involved in 1,8-cineole metabolism | 6-oxocineole + NAD(P)H | - |
r | |
1.1.1.241 | 6-endo-hydroxycineole + NAD(P)+ | Rhodococcus sp. C1 | involved in 1,8-cineole metabolism | 6-oxocineole + NAD(P)H | - |
r | |
1.14.13.51 | 6-oxocineole + NADPH + O2 | Rhodococcus | grown on 1,8-cineole or succinate as carbon source | 1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O | this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one | ? | |
1.14.13.51 | 6-oxocineole + NADPH + O2 | Rhodococcus C1 | grown on 1,8-cineole or succinate as carbon source | 1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O | this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.241 | Rhodococcus sp. | - |
- |
- |
1.1.1.241 | Rhodococcus sp. C1 | - |
- |
- |
1.14.13.51 | Rhodococcus | - |
- |
- |
1.14.13.51 | Rhodococcus C1 | - |
- |
- |
1.14.14.108 | Pseudomonas putida | - |
- |
- |
1.14.14.108 | Pseudomonas putida C1 / ATCC 17453 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.51 | partial | Rhodococcus |
1.14.14.108 | - |
Pseudomonas putida |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.14.108 | (+)-bornane-2,5-dione + FMNH2 + O2 = (+)-5-oxo-1,2-campholide + FMN + H2O | mechanism | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.241 | 0.62 | - |
6-endo-hydroxycineole | Rhodococcus sp. |
1.1.1.241 | 1.08 | - |
6-oxocineole | Rhodococcus sp. |
1.14.13.51 | 0.001 | - |
succinate grown cells | Rhodococcus |
1.14.13.51 | 0.3 | 0.4 | 1,8-cineole-grown cells | Rhodococcus |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.241 | -20°C, after dialysis against 1 mM KH2PO4/Na2HPO4 buffer, pH 7.1 | Rhodococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.241 | 6-endo-hydroxycineole + NAD(P)+ | 10% of activity with NADPH over NADH in reverse reaction | Rhodococcus sp. | 6-oxocineole + NAD(P)H | - |
r | |
1.1.1.241 | 6-endo-hydroxycineole + NAD(P)+ | involved in 1,8-cineole metabolism | Rhodococcus sp. | 6-oxocineole + NAD(P)H | - |
r | |
1.1.1.241 | 6-endo-hydroxycineole + NAD(P)+ | 10% of activity with NADPH over NADH in reverse reaction | Rhodococcus sp. C1 | 6-oxocineole + NAD(P)H | - |
r | |
1.1.1.241 | 6-endo-hydroxycineole + NAD(P)+ | involved in 1,8-cineole metabolism | Rhodococcus sp. C1 | 6-oxocineole + NAD(P)H | - |
r | |
1.14.13.51 | 6-oxocineole + NADPH + O2 | - |
Rhodococcus | 1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O | this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one | ? | |
1.14.13.51 | 6-oxocineole + NADPH + O2 | grown on 1,8-cineole or succinate as carbon source | Rhodococcus | 1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O | this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one | ? | |
1.14.13.51 | 6-oxocineole + NADPH + O2 | - |
Rhodococcus C1 | 1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O | this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one | ? | |
1.14.13.51 | 6-oxocineole + NADPH + O2 | grown on 1,8-cineole or succinate as carbon source | Rhodococcus C1 | 1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O | this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one | ? | |
1.14.13.51 | additional information | no substrates: substrate analogues, i.e. (+)-camphor, (S)-(+)-carvone, cyclohexanene, 1,3-cyclohexanedione, dihydrocarvone, 2-, 3-, and 4-methylcyclohexanone, fenchone, menthone, pinan-3-one, pulegone, verbenone | Rhodococcus | ? | - |
? | |
1.14.13.51 | additional information | no substrates: substrate analogues, i.e. (+)-camphor, (S)-(+)-carvone, cyclohexanene, 1,3-cyclohexanedione, dihydrocarvone, 2-, 3-, and 4-methylcyclohexanone, fenchone, menthone, pinan-3-one, pulegone, verbenone | Rhodococcus C1 | ? | - |
? | |
1.14.14.108 | (+)-bornane-2,5-dione + FMNH2 + O2 | i.e. 2,5-diketocamphane | Pseudomonas putida | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | i.e. cyclopentenoic acid | ? | |
1.14.14.108 | (+)-bornane-2,5-dione + FMNH2 + O2 | i.e. 2,5-diketocamphane | Pseudomonas putida C1 / ATCC 17453 | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | i.e. cyclopentenoic acid | ? | |
1.14.14.108 | (+)-bornanone + FMNH2 + O2 | (+)-camphor | Pseudomonas putida | 1,2-campholide + FMN + H2O | - |
? | |
1.14.14.108 | (+)-bornanone + FMNH2 + O2 | (+)-camphor | Pseudomonas putida C1 / ATCC 17453 | 1,2-campholide + FMN + H2O | - |
? | |
1.14.14.108 | 1,3,3-trimethyl-2-oxabicyclo-(2,2,2)octane + NADH + O2 | 6-oxocineole | Pseudomonas putida | 1,6,6-trimethyl-2,7-dioxa(3,2,2)bicyclononan-3-one + NAD+ + H2O | - |
? | |
1.14.14.108 | 1,3,3-trimethyl-2-oxabicyclo-(2,2,2)octane + NADH + O2 | 6-oxocineole | Pseudomonas putida C1 / ATCC 17453 | 1,6,6-trimethyl-2,7-dioxa(3,2,2)bicyclononan-3-one + NAD+ + H2O | - |
? | |
1.14.14.108 | 6-oxocineole + FMNH2 + O2 | - |
Pseudomonas putida | 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid + FMN + H2O | - |
? | |
1.14.14.108 | 6-oxocineole + FMNH2 + O2 | - |
Pseudomonas putida C1 / ATCC 17453 | 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid + FMN + H2O | - |
? | |
1.14.14.108 | additional information | rubredoxin not mentioned | Pseudomonas putida | ? | - |
? | |
1.14.14.108 | additional information | rubredoxin not mentioned | Pseudomonas putida C1 / ATCC 17453 | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.241 | 7.5 | - |
NADH oxidation | Rhodococcus sp. |
1.1.1.241 | 10.5 | - |
NAD+ reduction | Rhodococcus sp. |
1.14.13.51 | 9 | - |
- |
Rhodococcus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.241 | NAD+ | NADH in reverse reaction | Rhodococcus sp. | |
1.1.1.241 | NADP+ | NADPH in reverse reaction, 10% of activity over NADH | Rhodococcus sp. | |
1.14.13.51 | NADP+ | - |
Rhodococcus | |
1.14.13.51 | NADPH | - |
Rhodococcus | |
1.14.14.108 | FMN | - |
Pseudomonas putida |