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Literature summary extracted from

  • Wittenberger, C.L.
    D(-)-Lactate dehydrogenase from butyribacterium rettgeri (1975), Methods Enzymol., 41B, 299-303.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.28 repeated freezing and thawing, low ionic strength or pH above 6.5 cause loss of activity Eubacterium limosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.28 ATP
-
 Eubacterium limosum
1.1.1.28 Hg2+
-
 Eubacterium limosum
1.1.1.28 oxamate
-
 Eubacterium limosum
1.1.1.28 p-chloromercuriphenyl sulfonic acid
-
 Eubacterium limosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.28 145000
-
 gel filtration Eubacterium limosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+ Eubacterium limosum
-
 pyruvate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Eubacterium limosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.28
-
 Eubacterium limosum

Storage Stability

EC Number Storage Stability Organism
1.1.1.28 0°C or -20°C, 0.5 M potassium phosphate buffer pH 6.2, two weeks Eubacterium limosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+
-
 Eubacterium limosum pyruvate + NADH
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.28 5.8 6.5
-
 Eubacterium limosum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 NAD+
-
 Eubacterium limosum
1.1.1.28 NADH
-
 Eubacterium limosum