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Literature summary extracted from

  • Kochhar, S.; Chuard, N.; Hottinger, H.
    Cloning and overexpression of the Lactobacillus bulgaricus NAD(+)-dependent D-lactate dehydrogenase gene in Escherichia coli: purification and characterization of the recombinant enzyme (1992), Biochem. Biophys. Res. Commun., 185, 705-712.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.28 expression in Escherichia coli Lactobacillus delbrueckii subsp. bulgaricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.28 1.5 1.6 pyruvate recombinant and wild-type enzyme Lactobacillus delbrueckii subsp. bulgaricus
1.1.1.28 7.5 9 oxaloacetate recombinant and wild-type enzyme Lactobacillus delbrueckii subsp. bulgaricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.28 37000
-
 2 * 37000, SDS-PAGE Lactobacillus delbrueckii subsp. bulgaricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+ Lactobacillus delbrueckii subsp. bulgaricus
-
 pyruvate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Lactobacillus delbrueckii subsp. bulgaricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.28
-
 Lactobacillus delbrueckii subsp. bulgaricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.28 1950 2100
-
 Lactobacillus delbrueckii subsp. bulgaricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+
-
 Lactobacillus delbrueckii subsp. bulgaricus pyruvate + NADH
-
 ?
1.1.1.28 oxaloacetate + NADH
-
 Lactobacillus delbrueckii subsp. bulgaricus malate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.28 dimer 2 * 37000, SDS-PAGE Lactobacillus delbrueckii subsp. bulgaricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.28 7 7.5
-
 Lactobacillus delbrueckii subsp. bulgaricus